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Blood plasma extract for improving memory and preparation method and application of blood plasma extract

A technology to enhance memory and extracts, applied in the field of molecular biology, to achieve the effect of improving curative effect, improving cognitive level, improving and treating Alzheimer's disease

Active Publication Date: 2017-03-22
北京豪思生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the specific components of these substances and the methods of separation and acquisition have not been reported yet.

Method used

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  • Blood plasma extract for improving memory and preparation method and application of blood plasma extract
  • Blood plasma extract for improving memory and preparation method and application of blood plasma extract
  • Blood plasma extract for improving memory and preparation method and application of blood plasma extract

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preparation example Construction

[0052] Secondly, the present invention provides a method for preparing the plasma extract plasma extract HC4201614, the plasma extract is prepared from plasma extraction, and the preparation method includes the following steps in sequence: plasma collection, molecular weight cut-off, SD inactivation, centrifugation , anion exchange, dialysis, and concentration steps; preferably, after the plasma collection step and before the molecular weight cut-off step, the preparation method further includes a low-temperature filtration step and a low-temperature ultrafiltration step. details as follows:

[0053] (1) Plasma collection:

[0054] The blood is collected with an anticoagulant tube, and the supernatant is collected by centrifugation to obtain plasma; preferably, the centrifugal force is 500-1000 g, the centrifugation time is 10-20 min, and the centrifugation temperature is 0-15° C.

[0055] In an embodiment of the present invention, the above-mentioned centrifugal force can be...

Embodiment 1

[0101] The present embodiment is the preparation method of plasma extract HC4201614, and this method comprises the following steps:

[0102] (1) Collection of plasma

[0103] The blood of healthy people is obtained from blood donation in the hospital. The blood collection tube is an anticoagulant tube. During the blood collection process, the blood collection tube is shaken while bleeding to prevent blood coagulation; put the blood collection tube into a centrifuge, set the centrifugal force to 800g, and centrifuge for 15 minutes , and the centrifugation temperature was 4°C; then the supernatant was carefully sucked out with a pipette, which was the collected human plasma.

[0104] (2) Low temperature filtration:

[0105] The collected plasma was added to a filter with a filter membrane pore size of 0.22 microns, and a peristaltic pump was used to apply a pressure of 10 MPa for filtration. During the process, the temperature of the entire filtration device was controlled at 0...

Embodiment 2

[0151] In this embodiment, except step (9) is different from embodiment 1, other preparation steps are all identical with embodiment 1, and in this embodiment, step (9) is specifically as follows:

[0152] Add the dialysis product prepared in step (8) of Example 1 into the concentrating cup that allows 2.5kD substances to pass through, cover the concentrating cup, put the concentrating cup into the chromatographic cabinet, then connect the concentrating cup to the liquid nitrogen bottle, and open the The gas valve of the liquid nitrogen bottle is set at a pressure of 10MPa; under this pressure, the concentration cup starts to concentrate the product after dialysis, and when the volume of the concentrated product is observed to be 1 / 50 of that before concentration, the concentration is stopped and the concentrated product is obtained, which is Plasma extract HC4201614.

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Abstract

The invention belongs to the field of molecular biology, and provides a blood plasma extract for improving memory and a preparation method and application of the blood plasma extract. The blood plasma extract comprises various proteins and various micromolecules; SDS-PAGE deratarared gel electrophoresis of the blood plasma extract at least comprises five strips which are visible clearly by naked eyes; and the molecular weights of the strips are respectively 25 kD, 35 kD, 50 kD, 69 kD and 105 kD. The preparation method of the blood plasma extract comprises the following steps: blood plasma collecting, molecular weight interception, SD inactivation, centrifugal separation, anion exchange, dialysis and concentrating. The blood plasma extract prepared by the invention can be used for preventing, improving and treating senile dementia. In addition, the effect of improving memory can further be improved.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a plasma extract, in particular to a mixture separated from plasma for enhancing memory and a preparation method thereof, the mixture can be used to prevent, improve and treat Alzheimer's disease , and has the effect of enhancing memory. Background technique [0002] Alzheimer's disease (Alzheimer's disease, AD), also known as senile dementia, is a common type of chronic and progressive neurodegenerative disease in old age, which is characterized by the gradual loss of memory and cognitive ability of patients. . Patients die within 3 to 9 years of diagnosis, accounting for 50% to 56% of clinical deaths. The current understanding of the cause of the disease is the accumulation of abnormally folded beta-amyloid and tau proteins in the brain that lead to Alzheimer's disease. [0003] The medicines currently on the market for senile dementia are mainly western medicines, which are ch...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K9/00A61K45/00A61P25/28
CPCA61K9/0002A61K35/16A61K45/00
Inventor 崔文宏张丽丽孔毅荣郝敬雨石浩威任园园
Owner 北京豪思生物科技股份有限公司
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