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Chinese herbal medicine processing mechanism studying method

A technology of traditional Chinese medicine and mechanism, which is applied in the field of research on the processing mechanism of traditional Chinese medicine, can solve the problems of weak content and difficult to explore the interaction relationship, and achieve the effect of improving clinical curative effect

Active Publication Date: 2017-03-15
JIUJIANG UNIVERSITY
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  • Application Information

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Problems solved by technology

[0005] 3. The contribution of the blood-entering components of traditional Chinese medicine to its curative effect can be divided into main, secondary and auxiliary, that is, it also has an interaction relationship similar to that of "jun, minister, adjuvant and envoy" in traditional Chinese medicine prescriptions, but because of its weak content, it can It is difficult to explore its interaction relationship using conventional pharmacological methods

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the present invention takes the following research steps:

[0022] 1 Process the traditional Chinese medicine according to the "Chinese Pharmacopoeia", accurately weigh the processed Chinese medicine, extract it with 75% ethanol at a ratio of material to liquid of 1:8 for 2-4 hours, evaporate under reduced pressure until 1ml is equivalent to 6g of the processed Chinese medicine.

[0023] 2 Determination of the optimal blood collection time: 12 SD rats (180-220 g) were randomly divided into two groups, 6 in each group, and one group was the blank control group, and the same amount of distilled water 1mL was given according to the dose of the experimental group , 6 SD rats in the experimental group were administered with a dose of 30g of crude drug per kilogram of body weight, and blood was taken from the ocular venous plexus respectively after 30, 60, and 90 minutes after administration, and after each group was mixed, centrifuged at 3000 rpm for 10 minutes ...

Embodiment 2

[0029] Embodiment 2: the present invention takes the following research steps:

[0030] 1 Process the traditional Chinese medicine according to the "Chinese Pharmacopoeia", accurately weigh the processed Chinese medicine, extract it with 75% ethanol at a ratio of material to liquid of 1:8 for 2-4 hours, evaporate under reduced pressure until 1ml is equivalent to 6g of the processed Chinese medicine.

[0031] 2 Determination of the optimal blood collection time: 12 SD rats (180-220 g) were randomly divided into two groups, 6 in each group, and one group was the blank control group, and the same amount of distilled water 1mL was given according to the dose of the experimental group , 6 SD rats in the experimental group were administered with a dose of 30g of crude drug per kilogram of body weight, and blood was taken from the ocular venous plexus respectively after 30, 60, and 90 minutes after administration, and after each group was mixed, centrifuged at 3000 rpm for 10 minutes ...

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Abstract

Disclosed is a Chinese herbal medicine processing mechanism studying method. The Chinese herbal medicine processing mechanism studying method aims at key problems of Chinese herbal medicine processing mechanism study and comprises establishing blood-absorbing components of processed Chinese herbal medicine as a study target, identifying and online-analyzing the blood-absorbing components of the processed Chinese herbal medicine through serum pharmacochemical study and the computerized virtual screening technology, and studying the assistant and guide relations of the blood-absorbing components by combining fuzzy mathematical methods; comparing the change of the blood-absorbing components and the content of the blood-absorbing components before and after processing to outline the Chinese herbal medicine processing mechanism from the angle of blood-absorbing component change. Compared with existing Chinese herbal medicine processing mechanism study, the Chinese herbal medicine processing mechanism studying method is more intuitive in result due to the fact that study is performed from the angle of change of the blood-absorbing components with direct clinical effects, further, the change of medicinal material basis before and after processing is outlined and accordingly the processing mechanism can be outlined. The Chinese herbal medicine processing mechanism studying method outlines the traditional Chinese herbal medicine processing mechanism from a new angle and accordingly provides the basis for stability and controllability of quality of processed Chinese herbal medicine products.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a new method for research on the processing mechanism of traditional Chinese medicine. [0002] technical background [0003] 1. It is the tradition and characteristic of traditional Chinese medicine to use Chinese medicine after processing. After thousands of years of clinical application of processed traditional Chinese medicine, the effectiveness and necessity of its processing have been confirmed. The change of pharmacological activity of traditional Chinese medicine after processing is fundamentally based on the change of its active ingredients. Therefore, exploring the change of active ingredients is the key to the research of the processing mechanism of traditional Chinese medicine. The separation method can preliminarily clarify the chemical components of traditional Chinese medicine, but it is not clear whether these components that change after proces...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘可越刘海军
Owner JIUJIANG UNIVERSITY
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