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High-concentration microbial inoculum containing polyglutamic acid and preparation method and application of high-concentration microbial inoculum

A microbial agent, polyglutamic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inconvenient use, inconvenient transportation, single variety, etc.

Inactive Publication Date: 2017-03-15
DEZHOU YUANHE AGRI TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, in view of the shortcomings of microbial fertilizers such as low bacterial concentration, single variety, low soil colonization rate, inconvenient use, and inconvenient transportation, the number of effective viable bacteria obtained by the method described in the above patent has been improved to a certain extent compared with the current actual production. But there is still room for improvement, how to obtain a stable higher concentration of microbial inoculum is the main problem to be solved by the present invention

Method used

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  • High-concentration microbial inoculum containing polyglutamic acid and preparation method and application of high-concentration microbial inoculum
  • High-concentration microbial inoculum containing polyglutamic acid and preparation method and application of high-concentration microbial inoculum
  • High-concentration microbial inoculum containing polyglutamic acid and preparation method and application of high-concentration microbial inoculum

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preparation example Construction

[0020] The preparation method of the above-mentioned high-concentration microbial bacterial agent containing polyglutamic acid comprises the following steps: mixing mixed microbial fermentation powder, light calcium carbonate, polyglutamic acid, magnesium sulfate, boric acid, and zinc sulfate in proportion to obtain the product.

[0021] Preferably according to the present invention, mixed microbial fermentation powder is prepared as follows:

[0022] (1) After culturing Bacillus subtilis on a slant, the slant species A is obtained; the slant species A is transferred to a liquid seed medium, and cultured in a shake flask to obtain the liquid seed A;

[0023] The prepared liquid seed A is inoculated in the liquid fermentation medium, the pH value is controlled to 7-8, the aeration ratio is 1: (0.5-1), the rotation speed is 100-200 rpm, and cultured at 30-37°C for 36-48h , to obtain fermented liquid A; then fermented liquid A is transferred to a solid fermentation medium at a ma...

specific Embodiment approach

[0049] The technical solutions of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0050] microbial source

[0051] Bacillus subtilis (Bacillus subtilis) comes from the China Agricultural Microorganism Culture Collection and Management Center, and the strain number is ACCC 10242;

[0052] Bacillus laterosporus (Bacillus laterosporus) was obtained from the China Agricultural Microorganism Culture Collection and Management Center, and the strain number was ACCC 11079.

[0053] Bacillus licheniformis (Bacillus licheniformis) comes from the China Agricultural Microorganism Culture Collection Management Center, the strain number is ACCC 02002

[0054] Bacillus mucilaginosus (Paenibacillus mucilaginosus) comes from ACCC 10012, China Agricultural Microorganism Culture Collection Management Center

[0055] Bacillus megaterium (Bacillus megaterium) comes from the China I...

Embodiment 1

[0057] A high-concentration microbial bacterial agent containing polyglutamic acid, the components are as follows, all in parts by weight:

[0058] 20 parts of mixed microbial fermentation powder, 63 parts of light calcium carbonate, 8 parts of polyglutamic acid, 4 parts of magnesium sulfate, 3 parts of boric acid, and 2 parts of zinc sulfate;

[0059] The high-concentration microbial bacterial agent containing polyglutamic acid contains Bacillus subtilis, Bacillus laterosporus, Bacillus licheniformis, Paenibacillus mucilaginosus, Bacillus megaterium (Bacillus megaterium) The ratio of the number of live bacteria is 1:2.5:0.8:1:2.5, and the total number of effective live bacteria can reach 130 billion / gram.

[0060] The preparation method of the above-mentioned high-concentration microbial bacterial agent containing polyglutamic acid comprises the following steps:

[0061] (1) After culturing Bacillus subtilis on a slant, the slant species A is obtained; the slant species A is...

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Abstract

The invention relates to a high-concentration microbial inoculum containing polyglutamic acid and a preparation method and application of the high-concentration microbial inoculum. The high-concentration microbial inoculum containing polyglutamic acid is prepared from mixed microbial fermentation powder, light calcium carbonate, polyglutamic acid, magnesium sulfate, boric acid and zinc sulfate. Polyglutamic acid is added in the microbial inoculum, since polyglutamic acid contains a large number of super hydrophilic groups, namely, carboxyl groups, therefore, the microbial inoculum can keep the soil moisture, improve the porosity and bulking intensity of the soil, improve the water-retention capacity and fertilizer-rentention capacity of the soil and enhance the phosphorus releasing ability and the potassium releasing ability, and at the same time, the microbial colonization rate can be greatly improved by adding polyglutamic acid in the microbial inoculum.

Description

technical field [0001] The invention relates to a high-concentration microbial bacterial agent containing polyglutamic acid, a preparation method and application thereof, and belongs to the technical field of microbial bacterial agents. [0002] technical background [0003] China is a traditional large agricultural country. Agriculture is the basic industry of our country and occupies a pivotal position in the national economy. Since the reform and opening up, my country's grain production has achieved significant growth. Modern agricultural technology has promoted the rapid development of the agricultural industry. Among them, the extensive use of chemical fertilizers is an important factor supporting the increase in my country's grain production. Long-term excessive use of chemical fertilizers has brought about increasingly serious problems such as environmental pollution, soil compaction, soil fertility decline, ecological deterioration and decline in the quality of agric...

Claims

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Application Information

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IPC IPC(8): C05G3/04C12N1/20C12R1/125C12R1/10C12R1/11C12R1/07C05G3/80
CPCC05D3/02C05G3/80C12N1/20C05D5/00C05D9/02C05F11/08
Inventor 展彬许鹏飞梁东明李环环杜绍国
Owner DEZHOU YUANHE AGRI TECH DEVCO
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