Online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as crude medicine
A technology of natural antioxidants and antioxidant activity, which is used in material separation, analytical materials, measurement devices, etc.
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[0028] The present invention will be further described below in conjunction with accompanying drawing.
[0029] (1) Preparation of papaya extract
[0030] Take 5g of fresh papaya sample, add 10mL of methanol and bathe in water at 60°C for 10min, then add 5mL of water and mix well, then homogenize and flash extract for 1min with a homogenizer. After standing still, the supernatant was passed through a 0.22 microporous membrane to obtain papaya extract for later use.
[0031] (2) Determination of antioxidant activity of papaya extract
[0032]Dissolve DPPH in methanol, dilute to a final concentration of 0.04 mg / mL, and store at 4°C in the dark. Take 50 μL of papaya samples and 100 μL of DPPH (0.04 mg / mL) solution, mix well and react at room temperature in the dark for 30 min. Afterwards, the absorbance of the mixed solution was measured at 517nm in a microplate reader and recorded as A 样品 , the control group replaced the extract with the sample extraction solvent, and the ab...
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