Kit, PCR (polymerase chain reaction) primer and nucleic acid hybridization membrane strip for folic acid metabolism genotyping

Abstract

The invention discloses a kit, a PCR (polymerase chain reaction) primer and a nucleic acid hybridization membrane strip for folic acid metabolism genotyping. The nucleic acid hybridization membrane strip comprises a substrate and specific polymorphic detection probes fixed to the substrate, the polymorphic detection probes are in one-to-one correspondence to folic acid metabolism related gene polymorphic sites, and the related gene polymorphic sites include at least one of a c.677 site and a c.1298 site of an MTHFR (methylene tetrahydrofolate reductase) gene, a c.56 site and a c.66 site of an MTRR (methionine synthase reductase) gene, a -186 site, a c.2756 site and a +905 site of an MTR (methionine synthase) gene and a -551 site of a CBS (cystathionine-beta-synthetase) gene. Each polymorphic detection probe comprises 13-25 base sequences. The kit, the PCR primer and the nucleic acid hybridization membrane strip are easy for judgment of polymorphic heterozygotes, polymorphic homozygotes and the like and have advantages of high flux, high efficiency, low cost, convenience in use and the like.

Description

technical field [0001] The invention relates to molecular biology detection, in particular to a nucleic acid hybridization membrane strip for evaluating folic acid metabolism and a polymerase chain reaction (Polymerase Chain Reaction, PCR) primer for amplifying gene fragments of samples to be tested; The present invention also relates to a kit for evaluating folic acid metabolism ability. Background technique [0002] Folic acid metabolism is closely related to many important biochemical processes. On the one hand, it can affect the synthesis of nucleic acids; has an extremely important role. Studies have shown that folic acid metabolism disorders can affect the synthesis and methylation of DNA and amino acids, and can lead to hyperhomocysteinemia, which is closely related to birth defects such as congenital heart disease, neural tube defects, and recurrent miscarriages . The factors of folate metabolism disorder are very complex, including lack of folic acid and methyl d...

AI Technical Summary

Problems solved by technology

[0025] At present, traditional gene diagnosis methods include enzyme digestion, restriction fragment length polymorphism (RFLP), direct sequencing, etc. These methods are either not qualitative, or time-consuming and labor-intensive, and require expensive equipment and consumables. Unfortunately, these methods are difficult to simultaneously detect multiple polymorphic sites in different genes

Although the gene chip method can detect different gene loci at the same time, it requires expensive equipment and consumables, and is not suitable for large-scale clinical promotion, and its application is limited.

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