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Small peptide chelated zinc as well as preparation method and application thereof

A technology of small peptide chelation and inorganic zinc, which is applied in the field of feed additives, can solve problems such as few reports, achieve the effects of reducing environmental hazards, prolonging shelf life, and realizing high-value processing

Inactive Publication Date: 2017-03-08
YATAI XINGYUAN FARMING TECH HAIAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports at home and abroad on the effects of small peptides with different molecular weights on piglet growth and feed shelf life under different chelation rates.

Method used

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  • Small peptide chelated zinc as well as preparation method and application thereof
  • Small peptide chelated zinc as well as preparation method and application thereof
  • Small peptide chelated zinc as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Take 2000g of miscellaneous fish as the protein raw material, crush the miscellaneous fish with a meat grinder, add deionized water according to the ratio of material to liquid (mass) 1:3, adjust the pH value to 5, heat up to 50°C, and add papain , Flavor protease (papain 100,000 U / g, flavor protease 15,000 U / g, compound ratio 1:1, enzyme amount equivalent to 0.5% of trash fish mass, Nanning Pangbo Bioengineering Co., Ltd.) enzymolysis to the degree of hydrolysis 30% (Tan Zhifen et al., the assay method of the degree of protein hydrolysis. Analysis and detection, 2005), inactivate the enzyme at 100°C for 20min, centrifuge at 6000rpm for 15min, first ultrafilter with 10kDa ultrafiltration membrane, and then obtain the permeate Use 1kDa ultrafiltration, the non-permeable liquid is the trash fish protein hydrolyzate with a molecular weight between 1 and 10kDa (named A ultrafiltration protein hydrolyzate), and the permeated liquid is the trash fish protein hydrolyzate wi...

Embodiment 2

[0038] (1) Take 2000g of miscellaneous fish as the protein raw material, crush the miscellaneous fish with a meat grinder, add deionized water according to the ratio of material to liquid (mass) 1:4, adjust the pH value to 6, heat up to 60°C, add neutral Protease, flavor protease (neutral protease 300,000 U / g, flavor protease 15,000 U / g, compound ratio 1:1, the amount of enzyme added is equivalent to 0.3% of the mass of trash fish, Nanning Pangbo Bioengineering Co., Ltd.) enzymolysis to The degree of hydrolysis is 30% (Tan Zhifen et al., 2005), inactivate the enzyme at 100°C for 20 minutes, centrifuge at 6000rpm for 15 minutes, first use 10kDa ultrafiltration membrane ultrafiltration, then 1kDa ultrafiltration, and then use 1kDa ultrafiltration to obtain the permeate Ultrafiltration, non-permeable liquid is trash fish protein hydrolyzate with molecular weight between 1 and 10kDa (named A ultrafiltration protein hydrolyzate), and permeated liquid is trash fish protein hydrolyzat...

Embodiment 3

[0041](1) Take 2000g of miscellaneous fish as the protein raw material, crush the miscellaneous fish with a meat grinder, add deionized water according to the ratio of material to liquid (mass) 1:5, adjust the pH value to 7, heat up to 70°C, add neutral Protease, papain (neutral protease 300,000 U / g, papain 100,000 U / g, compound ratio 1:1, the amount of enzyme added is equivalent to 0.1% of the mass of miscellaneous fish, Nanning Pangbo Bioengineering Co., Ltd.) enzymolysis to The degree of hydrolysis is 30% (Tan Zhifen et al., 2005), inactivate the enzyme at 100°C for 20 minutes, centrifuge at 6000rpm for 15 minutes, first use 10kDa ultrafiltration membrane for ultrafiltration, and then use 1kDa to obtain the permeate. Then use 1kDa ultrafiltration, the non-permeable liquid is the trash fish protein hydrolyzate with a molecular weight between 1 and 10kDa (named A ultrafiltration protein hydrolyzate), and the permeated liquid is the trash fish protein hydrolyzate with a molecul...

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Abstract

The invention discloses small peptide chelated zinc as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing coarse fishes and adding water; adjusting the pH (Potential of Hydrogen) to 5-9; heating and raising the temperature to 50 DEG C-70 DEG C; adding proteinase for enzymolysis; carrying out the enzymolysis until the hydrolysis degree is 30%; heating and carrying out enzyme inactivation and centrifuging; carrying out ultra-filtration by utilizing a 10kDa ultra-filtration membrane and taking permeate liquid; carrying out the ultra-filtration on the permeate liquid by utilizing a 1kDa ultra-filtration membrane, and taking non-permeate liquid; adding inorganic zinc salt into the non-permeate liquid, and stirring and oscillating under the conditions that the temperature is 40 DEG C-60 DEG C and the pH value is 5-7 until the chelating rate is 70%+ / -5%; centrifuging and concentrating; and freeze-drying to obtain the small peptide chelated zinc. According to the small peptide chelated zinc disclosed by the invention, the digestion and absorption of proteins and microelements of piglets are improved and the immunity of the piglets is enhanced; the small peptide chelated zinc also has good anti-oxidization and antibacterial functions and is added into feed so that the guarantee period of the feed can be effectively prolonged; and risks that the diarrhea of the piglets is caused and the immunity is reduced and the like, caused by the fact that the feed goes bad, are reduced.

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to a small peptide chelated zinc and its preparation method and application. Background technique [0002] Due to the perishability and low processing value of marine low-value trash fish, fishermen lack enthusiasm for this kind of fish, which makes this large class of fish species fend for itself, resulting in a great waste of natural resources. However, marine low-value small trash fish are rich in protein, which can be enzymatically hydrolyzed in batches and chelated with trace elements to prepare feed additives with anti-mold and anti-oxidation, so as to improve the high-value processing level of trash fish and prevent trash fish from rotting and deteriorating harm to the environment. [0003] Zinc is one of the essential trace elements for animals. It has been compared to the "life element" of animals. Zinc is a component of more than 200 metalloenzymes, hormones and insulin in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23K20/147A23K50/30
CPCC12P21/06C07K1/34
Inventor 李同刚陈志敏李炯明周盛昌肖丽萍施建成吴有林
Owner YATAI XINGYUAN FARMING TECH HAIAN CO LTD
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