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Preparation and application method of immobilized agriculture microbial agent

A technology of microbial agent and application method, which is applied in the field of fertilizer production, can solve the problems of high process cost, unfavorable large-scale promotion, slow degradation of polymer compounds, environmental pollution, etc., to meet the needs of growth and reproduction, service life and application effect enhancement , the effect of no pollution to the environment

Inactive Publication Date: 2017-03-08
YANTAI GOODLY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immobilization or embedding method is conducive to the formation of local growth advantages of microorganisms, but the high cost of the process is not conducive to large-scale promotion
At the same time, some polymer compounds degrade slowly in the soil, which is easy to pollute the environment
There is no report on the use of sea oyster shells and kelp roots as microbial immobilization carriers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of preparation and application of immobilized agricultural microbe bacterial agent, comprises the following steps:

[0027] 1) Add 5 times the volume of water to the sea oyster shell, add citric acid to adjust the pH value to 3.5, soak for 2 hours, pulverize, ventilate and dry, control the moisture to not exceed 10%, and pass through a 80-100 mesh sieve to obtain sea oyster shell powder ;

[0028] 2) Add 4 times potassium carbonate solution to the kelp root, the solution concentration is 1.0mol / L, soak at 60°C for 3 hours, pulverize, ventilate and dry, control the water content to not exceed 5%, and pass through a 80-100 mesh sieve to obtain the kelp root powder;

[0029] 3) Mix sea oyster shell powder and seaweed root powder evenly in a ratio of 2:1;

[0030] 4) Mix Bacillus subtilis and Bacillus licheniformis at a ratio of 1:1 to make a bacterial suspension, and control the effective concentration of viable bacteria to 120 billion / ml. Step 3) Add the mixture t...

Embodiment 2

[0034] A kind of preparation and application of immobilized agricultural microbe bacterial agent, comprises the following steps:

[0035] 1) Add 8 times the volume of water to the sea oyster shell, add malic acid to adjust the pH value to 4.0, soak for 2 hours, pulverize, ventilate and dry, control the moisture to not exceed 10%, and pass through a 80-100 mesh sieve to obtain sea oyster shell powder ;

[0036] 2) Add 5 times sodium bicarbonate solution to the kelp root, the solution concentration is 1.2mol / L, soak at 65°C for 3 hours, pulverize, ventilate and dry, control the water content to not exceed 5%, and pass through a 80-100 mesh sieve to obtain the kelp root powder;

[0037] 3) Mix sea oyster shell powder and kelp root powder evenly in a ratio of 3:1;

[0038] 4) Make a bacterial suspension of Bacillus subtilis and Bacillus megaterium at a ratio of 5:1, control the effective concentration of viable bacteria to 150 billion / ml, add the mixture in step 3) to the bacteri...

Embodiment 3

[0042] A kind of preparation and application of immobilized agricultural microbe bacterial agent, comprises the following steps:

[0043] 1) Add 10 times the volume of water to the sea oyster shell, add oxalic acid to adjust the pH value to 4.0, soak for 2 hours, pulverize, ventilate and dry, control the moisture to not exceed 10%, and pass through a 80-100 mesh sieve to obtain sea oyster shell powder;

[0044] 2) Add 8 times potassium carbonate solution to the kelp root, the solution concentration is 1.0mol / L, soak at 65°C for 3 hours, pulverize, ventilate and dry, control the water content to not exceed 5%, and pass through a 80-100 mesh sieve to obtain the kelp root powder;

[0045] 3) Mix sea oyster shell powder and seaweed root powder evenly in a ratio of 2:1;

[0046] 4) Prepare Bacillus lateralis, Bacillus licheniformis, and Bacillus jelly-like in a ratio of 1:2:1 to make a bacterial suspension, control the effective concentration of viable bacteria to 160 billion / ml, a...

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PUM

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Abstract

The invention relates to a preparation and application method of an immobilized agricultural microbial agent. The immobilized agricultural microbial agent is particularly characterized by being prepared from a mixture of oyster shells and seaweed roots. The method comprises the following steps: (1) soaking the oyster shell in a weak acid solution, and crushing, drying and sieving to obtain oyster shell powder; (2) dipping the kelp roots in an alkaline solution, and crushing, drying and sieving to obtain kelp root powder; (3) mixing the oyster shell powder with the kelp root powder according to a ratio of (2 to 1) to (3 to 1); (4) adding the mixture obtained in step (3) into a bacterial suspension at a bathing temperature of 40 DEG C to 60 DEG C, and stirring for 2 hours; and (5) performing natural sedimentation at room temperature for 0.5 to 1.5 hours, separating solids from liquid, and drying to obtain the agricultural microbial agent which is stable in effect, long in effective period and high in environmental applicability.

Description

technical field [0001] The invention relates to the preparation and application of an immobilized agricultural microbial agent, belonging to the technical field of fertilizer production. Background technique [0002] As we all know, my country's agricultural production has been overly dependent on chemical fertilizers for a long time, resulting in the waste of a large amount of non-renewable energy, the deterioration of farmland soil quality, the decline of fertility, the degradation of crop quality, and the increasingly serious problems of food and environmental pollution. Microbial fertilizers can improve the nutritional environment of the rhizosphere, decompose insoluble phosphorus and potassium compounds in the soil, reduce the use of chemical fertilizers and improve crop quality. The development of efficient, stable and safe compound bio-fertilizers is of great significance to the future development of my country's agriculture. [0003] Prolonging the shelf life of fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02
CPCC12N11/02
Inventor 常大勇常晓菲高筱莎
Owner YANTAI GOODLY BIOTECH
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