A kind of biologically inducible artificial dura mater and preparation method thereof

A dura mater and sealing membrane technology, applied in the field of biologically induced artificial dura mater and its preparation, can solve the problems of easy tearing, cerebrospinal fluid leakage, easy breakage, etc., and achieve good tensile strength and mechanical properties, good tearing Strength and handling characteristics, the effect of preventing sticking effect

Active Publication Date: 2020-01-21
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this material is a derivative of skin. Natural skin has pores, sweat glands, blood vessels, and collagen fiber gaps, etc., and has a natural pore structure. Some products have large pores, which may cause cerebrospinal fluid leakage.
[0006] All in all, among the current dural repair materials, the silk fibroin film still needs to be further improved in the production process; the amniotic membrane is worthy of further exploration in terms of the sampling method and the selection of cross-linking agents; the biological toxicity and physical and chemical properties of polymer materials are also important. Long-term experiments are yet to be confirmed; research on bacterial cellulose membranes in the field of dura mater has not been reported in the literature; existing collagen membranes, collagen sponges, etc. still have problems such as the degradation rate is not easy to control, so that new meninges have not yet formed after material degradation. The material is hard and easily broken, and it is easy to tear when sutured, causing cerebrospinal fluid leakage

Method used

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  • A kind of biologically inducible artificial dura mater and preparation method thereof
  • A kind of biologically inducible artificial dura mater and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Preparation of decellularized porcine dermis: split the ecological pigskin into layers, use disinfection, surface cleaning and other technologies to remove the heat source of raw materials; use Pingpingjia, ethanol and other reagents for degreasing treatment; use compound enzyme decellularization process and Triton-100 cleaning The method of combining techniques removes cell components; the obtained decellularized porcine dermal matrix is ​​further treated with depyrogenation by using an acid-base combination method; freeze-dried for future use;

[0050] (2) Polyethylene glycol and glycerin carry out copolymerization and cross-linking reaction to obtain a composite glue. Apply it on the decellularized dermal matrix by means of horizontal salivation and horizontal spraying, control the process parameters such as pressure, spraying distance, spraying rate, etc., so that the coating is evenly distributed, dry at room temperature, and dry and solidify at a constant tempe...

Embodiment 2

[0054] 1. Check the physical properties of the prepared materials

[0055] (1) Appearance: Visual observation and actual operation show that the collagen-based dura mater is in the shape of white flakes, with a certain hardness in the dry state, and soft and not easy to break in the wet state. .

[0056] (2) Size: Measured with a general measuring tool, the length and width of the collagen-based dura mater are 25mm-100mm, and the thickness is 0.3-0.7mm. .

[0057] (3) Absorption: When tested according to the method in 3.2 of YY / T0471.1-2004, the absorption capacity per gram of sample

[0058] The liquid absorption is greater than 3g.

[0059] 4) Suture strength test: method: use 4-0 surgical suture to suture at the edge of one end of the meningeal patch at 3 mm, fix the suture and the other end of the dura patch on the tensile instrument, and use AI-7000S electronic The tensile machine is stretched until the suture is torn, and the tension at which the suture is torn is re...

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Abstract

The invention provides a biologically-induced artificial dura mater and a preparation method thereof. The biologically-induced artificial dura mater has a dual-layer structure; a decellularized pig dermis scaffold is used as a substrate; and the decellularized pig dermis scaffold is uniformly coated by a collagen compound adhesive with excellent biocompatibility so as to form a film capable of preventing seepage of cerebrospinal fluids. The collagen fiber scaffold retains the three-dimensional structure of a natural matrix, maximally maintains a structure foundation, i.e., a three-strand spiral structure, capable of performing biological induction action, and guarantees biological activity. The collagen-based artificial dura mater prepared by using the method has excellent biocompatibility as collagen and good mechanical properties as decellularized pig dermis.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a biologically induced artificial dura mater and a preparation method thereof. Background technique [0002] The dura mater is a layer of hard membranous tissue between the inside of the skull and the brain tissue. It is composed of a collagen fiber network and fibroblasts entwined in it. It is close to the inside of the skull and its main function is to protect the brain. When a person suffers from an intracranial tumor, the dura mater tissue is easily eroded and damaged; in addition, dural defects may also be caused by surgery, trauma and other reasons. Dural defects can cause a series of complications such as brain iatrogenic injury, cerebrospinal fluid leakage, intracranial infection, and secondary epilepsy. Timely and effective dura mater repair is necessary to prevent adhesions between the brain and spinal cord and surrounding tissues, prevent cerebrospinal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/50
Inventor 宋强富勇马文杰彭红
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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