Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound antibody containing interferon alpha/tumor antibody/oxidized graphene as well as preparation method and applications

A technology of interferon alpha and graphene, applied in the field of antibodies, to achieve the effect of preventing recurrence, curing tumors, shrinking and clearing tumors

Active Publication Date: 2017-03-08
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a compound antibody comprising interferon alpha / tumor antibody / graphene oxide, its preparation method and application, so as to solve the technical problem that the existing immunotherapy is only effective for a small number of patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound antibody containing interferon alpha/tumor antibody/oxidized graphene as well as preparation method and applications
  • Compound antibody containing interferon alpha/tumor antibody/oxidized graphene as well as preparation method and applications
  • Compound antibody containing interferon alpha/tumor antibody/oxidized graphene as well as preparation method and applications

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] Another aspect of the present invention provides a method for preparing a composite antibody comprising interferon alpha / tumor antibody / graphene oxide, comprising the following steps:

[0043] The interferon α and graphene oxide were mixed and reacted at 32-42°C for 2-4 hours.

[0044] Add tumor antibody and mix well, and react at 32-42°C for 4-8 hours to obtain a composite antibody comprising interferon α / tumor antibody / graphene oxide.

[0045] Specifically, 0.2 mg of GO and 2000 units of INFα can be incubated at 37° C. for 2 hours with continuous stirring. Add 1mg of tumor antibody (RTX or TRA) to the prepared 1ml INFα / GO, incubate at 37°C for 12 hours with continuous stirring, and set aside. The combination sequence of the above three substances can ensure a relatively high combination amount of the three substances, so as to ensure the therapeutic effect.

[0046] Optionally, the reaction temperature of interferon α and graphite oxide is 37°C, and the reaction tim...

Embodiment 1

[0061] GO 0.2 mg and INFα 2000 units were incubated at 37°C for 2 hours with constant agitation. The prepared 1ml INFα / GO was added with 1mg RTX monoclonal antibody, incubated at 37°C for 12 hours with continuous stirring, and set aside. GO is graphene oxide that is ultrasonically crushed and filtered through a filter with a pore size of 0.22 μm.

Embodiment 2

[0063] GO 0.1 mg and INFα 2000 units were incubated at 37°C for 2 hours with constant agitation. The prepared 1ml INFα / GO was added with 5mg TRA monoclonal antibody, incubated at 37°C for 12 hours with continuous stirring, and set aside. GO is graphene oxide that is ultrasonically crushed and filtered through a filter with a pore size of 0.22 μm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a compound antibody containing interferon alpha / tumor antibody / oxidized graphene as well as a preparation method and applications. The compound antibody contains interferon alpha, the tumor antibody and oxidized graphene, wherein the interferon alpha, the tumor antibody and oxidized graphene are connected through non covalent bonds, and the tumor antibody is rituximab or trastuzumab. The compound antibody containing interferon alpha / tumor antibody / oxidized graphene can effectively and directly kill most tumor cells and damage the microenvironment protecting the tumor cells, and meanwhile, the interferon alpha stimulates an immune system, breaks the tumor tolerance, and promotes the specific cellular activation of tumors or the release of cell factors, so that the purposes of shrinking or eliminating tumors, preventing relapse and finally completely treating tumors are achieved.

Description

technical field [0001] The present invention relates to the field of antibodies, in particular, to a composite antibody comprising interferon alpha / tumor antibody / graphene oxide. In addition, the present invention also relates to a preparation method and application of a composite antibody comprising interferon alpha / tumor antibody / graphene oxide. Background technique [0002] Chemotherapy is a commonly used method for treating tumors. Although it has been reported in the literature that chemotherapy can reduce mortality, there are also reports in the literature that chemotherapy can neither increase the curative effect nor prolong life, because chemotherapy damages the immune system and loses the efficacy of tumor immunity. Over the past few decades, immunotherapy and targeted therapy have been two different effective strategies, each proven to have its own advantages and disadvantages. Identification of drivers of cancer by genome-guided mutations has facilitated the deve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/395A61K38/21A61K47/04A61P35/00
CPCA61K38/212A61K39/39558A61K47/02A61K2300/00
Inventor 罗承科陈新建王锡阳陶立坚黄菊芳李岚邓征浩
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com