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Cultivation method of moss and desert algae composite crust

A cultivation method and technology of moss crust, applied in the field of cultivation of moss and desert algae composite crust, can solve the problems such as difficulty in large-scale production, improve species diversity and viability, accelerate succession process, and improve substrate The effect of conditions and circumstances

Active Publication Date: 2017-03-08
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (application) No. 200810035554.x provides a method for the rapid proliferation of desert moss and its biological crusts. This technology can realize indoor small-scale cultivation of desert moss crusts, but it is difficult to achieve in large-scale production , but also not suitable for desertification areas

Method used

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  • Cultivation method of moss and desert algae composite crust
  • Cultivation method of moss and desert algae composite crust
  • Cultivation method of moss and desert algae composite crust

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] according to figure 1 As can be seen, a method for cultivating moss and desert algae composite crust, the steps are:

[0034] 1. Confirmation of moss dominant species in the desertification area to be rehabilitated 1 and collection and preservation of moss crusts 2: According to extensive investigations, identify the dominant moss species of biological soil crusts in the natural habitat of the area to be rehabilitated in summer, and select the well-developed moss species. For moss crusts, the superficial crusts were collected with a ring knife, and air-dried at room temperature for 10, 13, or 15 days for later use.

[0035] 2. Moss crust activity recovery 3: The activity of the moss crust is recovered. The air-dried moss samples are sprayed with water to be completely moistened and then cultured for at least 48 hours to recover their activity; 15 °C with light intensity of 5 or 10 or 15 or 20 μmol m -2 s -1 , the light-dark cycle is 12 / 12, and the humidity is 60 or ...

Embodiment 2

[0049] according to figure 1 As can be seen, a method for cultivating moss and desert algae composite crust, the steps are:

[0050] The investigation found that moss ( Bryum argenteum ) and P. terrestris ( Didymodon vinealis ) are widely distributed in Dalat Banner, Inner Mongolia Autonomous Region. Two well-developed moss crusts were selected and stored separately after air-drying. The air-dried moss samples were sprayed with water until completely wet, and the temperature was 15 °C and the light intensity was 20 μmol m -2 s -1 , with a light-dark cycle of 12 / 12 and a humidity of 80% for at least 48 hours to recover its activity. After recovery, the moss crusts were washed with distilled water to remove the sandy soil to obtain moss gametophytes. Gametocytes were washed three times in sterile distilled water. It was then disinfected with 5% sodium hypochlorite by mass for 10 seconds, and then washed three times with distilled water. The sterilized moss gametophyte...

Embodiment 3

[0057] The culture method of P. terrestris gametophyte is the same as that in Example 1.

[0058] Microsphingomyelinae and Pseudomonas javanica were cultured separately ( Scytonema javanicum ) to the logarithmic phase, and the culture method was the same as that in Example 1 or Example 2.

[0059] Mix the gametophyte of P. sylvatica with Microsphingoencephala and Pseudomonas javanica according to the dry weight ratio of 1:5:3, and then spray and inoculate on the surface of the sand after mixing evenly. and Pseudomonas javanica total dry weight of 0.9 g m -2 .

[0060] Maintenance after skin inoculation was the same as in Example 1 or Example 2.

[0061] After one month, the initial composite crust of Paradontia terrestris, Microsphingoencephala and Pseudomonas javanica can be formed, and the crust coverage can reach about 94%, of which the coverage of moss can reach 50%-92%.

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Abstract

The invention discloses a cultivation method of a moss and desert algae composite crust. The cultivation method comprises the first step of collecting in summer a superior kind of moss in a natural habitat of an area to be restored; the second step of returning activities of the moss; the third step of using distilled water to wash moss crust the activities of which are returned and obtaining moss gametophyte; the fourth step of putting the gametophyte into a solid culture medium to be subjected to cultivation after the gametophyte is subjected to sterilization and obtaining moss protonema; the fifth step of picking the moss protonema and conducting static liquid cultivation to obtain differentiated gametophyte, and putting the gametophyte into a fluid culture medium to conduct aerobic cultivation; the sixth step of conducting aerobic cultivation on the desert algae through the fluid culture medium till a logarithmic growth phase, and obtaining algae thick liquid after removing supernatant; the seventh step of mixing the moss gametophyte with the desert algae thick liquid and afterwards inoculating the moss gametophyte and the desert algae thick liquid to the surface of desertification soil, and cultivating the moss and desert algae composite crust. The cultivation method of the moss and desert algae composite crust is easy to carry out and convenient to operate, evolvement process of biological soil crust in a desert area is accelerated, and fast treatment of desertification soil in arid and semi-arid lands is realized.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering, relates to ecological restoration of desertified soil, and more particularly relates to a method for cultivating composite crusts of moss and desert algae. Background technique [0002] Biological soil crusts are topsoils formed by the preferential growth of algae, and after the soil surface is fixed, other cryptoflora plants also grow on it. They are widely distributed in arid and semi-arid regions of the world, where they play important ecological roles such as carbon sequestration, nitrogen fixation, soil stabilization, changes in soil reflectance and water conditions, and effects on vascular plant growth and seed germination. Biological soil crusts are usually composed of fungi, lichens, mosses and algae. According to the main constituent organisms in biological soil crusts, it can be divided into algal crusts, lichen crusts and moss crusts. Under normal circumstances, alg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G1/00C12N1/12C12R1/89
CPCA01G22/00A01G24/00A01G31/00C12N1/12
Inventor 李敦海彭成荣郑娇莉黄顺张金丽刘永定
Owner INST OF AQUATIC LIFE ACAD SINICA
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