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A kind of cultivation method of compound crust of moss and desert algae

A cultivation method and technology of moss crust, applied in the field of cultivation of moss and desert algae composite crust, can solve the problems such as difficulty in large-scale production, improve species diversity and viability, accelerate succession process, and improve substrate The effect of conditions and circumstances

Active Publication Date: 2019-07-05
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent (application) No. 200810035554.x provides a method for the rapid proliferation of desert moss and its biological crusts. This technology can realize indoor small-scale cultivation of desert moss crusts, but it is difficult to achieve in large-scale production , but also not suitable for desertification areas

Method used

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  • A kind of cultivation method of compound crust of moss and desert algae
  • A kind of cultivation method of compound crust of moss and desert algae
  • A kind of cultivation method of compound crust of moss and desert algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] according to figure 1 It can be seen that a method for cultivating the composite crust of moss and desert algae includes the following steps:

[0034] 1. Confirmation of dominant species of moss in desertification areas to be treated 1 and collection and preservation of moss crust 2: According to extensive surveys, identify the dominant species of moss with biological soil crusts in the natural habitat of the area to be restored in summer, and select the well-developed species For moss crusts, collect the surface crusts with a ring knife and air-dry for 10, 13 or 15 days at room temperature for later use.

[0035] 2. Moss crust activity recovery 3: The moss crust activity is restored. The air-dried moss sample is sprayed with water until it is completely wet and incubated for at least 48 hours to restore its activity; the temperature is set to 10 or 12 or 14 or during the activity recovery of the moss sample 15 °C, the light intensity is 5 or 10 or 15 or 20 μmol m -2 s -1 ,...

Embodiment 2

[0049] according to figure 1 It can be seen that a method for cultivating the composite crust of moss and desert algae includes the following steps:

[0050] The investigation found that the moss ( Bryum argenteum ) And the native pair of tooth moss ( Didymodon vinealis ) Widely distributed in Dalat Banner, Inner Mongolia Autonomous Region. Choose two well-developed moss crusts and store them separately after air-drying. The air-dried true moss sample is sprayed with water until it is completely wet, at a temperature of 15 °C and a light intensity of 20 μmol m -2 s -1 , The light-dark cycle is 12 / 12, and the humidity is 80% by incubating for at least 48 hours to restore its activity. After the activity is restored, the moss crust is washed off the sand with distilled water to obtain the moss gametophyte. The gametophyte was washed three times in sterile distilled water. Then disinfect with 5% sodium hypochlorite by mass for 10 seconds, and then rinse with distilled water thr...

Embodiment 3

[0057] The method of culturing the gametophytes of Pterodactylon dentatus is the same as in Example 1.

[0058] Separately cultivate Microcodone sheath and Pseudocladia javanica ( Scytonema javanicum ) To the log phase, the cultivation method is the same as in Example 1 or Example 2.

[0059] Combine the gametophytes of the terrestrial moss, Microcodonia and Pseudocladea javanica in a ratio of 1:5:3 with a dry weight of 1:5:3. After mixing, spray and inoculate on the surface of the sand. The total dry weight of Pseudocladus javanica is 0.9 gm -2 .

[0060] The maintenance of the crust after inoculation is the same as in Example 1 or Example 2.

[0061] One month later, the initial composite crusts of the terrestrial moss, Microcodyle and Pseudocladia javanica can be formed. The crust coverage can reach about 94%, and the coverage of moss can reach 50%-92%.

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Abstract

The invention discloses a cultivation method of a moss and desert algae composite crust. The cultivation method comprises the first step of collecting in summer a superior kind of moss in a natural habitat of an area to be restored; the second step of returning activities of the moss; the third step of using distilled water to wash moss crust the activities of which are returned and obtaining moss gametophyte; the fourth step of putting the gametophyte into a solid culture medium to be subjected to cultivation after the gametophyte is subjected to sterilization and obtaining moss protonema; the fifth step of picking the moss protonema and conducting static liquid cultivation to obtain differentiated gametophyte, and putting the gametophyte into a fluid culture medium to conduct aerobic cultivation; the sixth step of conducting aerobic cultivation on the desert algae through the fluid culture medium till a logarithmic growth phase, and obtaining algae thick liquid after removing supernatant; the seventh step of mixing the moss gametophyte with the desert algae thick liquid and afterwards inoculating the moss gametophyte and the desert algae thick liquid to the surface of desertification soil, and cultivating the moss and desert algae composite crust. The cultivation method of the moss and desert algae composite crust is easy to carry out and convenient to operate, evolvement process of biological soil crust in a desert area is accelerated, and fast treatment of desertification soil in arid and semi-arid lands is realized.

Description

Technical field [0001] The invention belongs to the technical field of environmental engineering, relates to the ecological restoration of desertified soil, and more specifically relates to a method for cultivating a composite crust of moss and desert algae. Background technique [0002] Biological soil crusts (Biological soil crusts) is the preferential growth of algae. After the soil surface is fixed, other cryptographic plants will then grow on the surface soil formed on it. They are widely distributed in arid and semi-arid regions around the world, where they play important ecological roles in fixing carbon, fixing nitrogen, stabilizing soil, changing soil reflectance and water conditions, and affecting the growth of vascular plants and seed germination. Biological soil crusts are usually composed of fungi, lichens, moss and algae. According to the different main organisms in biological soil crust, it can be divided into algal crust, lichen crust and moss crust. Under norma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G22/30C12N1/12C12R1/89
CPCA01G22/00A01G24/00A01G31/00C12N1/12
Inventor 李敦海彭成荣郑娇莉黄顺张金丽刘永定
Owner INST OF AQUATIC LIFE ACAD SINICA
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