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Pectase enzymolysis method of antibiotic residue

A technology of antibiotic bacteria residue and pectinase enzyme is applied in the field of enzymatic hydrolysis of antibiotic residues of antibiotic residues by using pectinase and enzymatic hydrolysis of antibiotic residues of antibiotic residues, which can solve the problems of long treatment period, high cost and complicated operation. , to achieve the effect of convenient subsequent processing, short reaction period and simple operation

Inactive Publication Date: 2017-03-01
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used to solve technical problems such as difficult treatment of antibiotic bacteria residue, high cost, easy to produce secondary pollution, long treatment cycle and complicated operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Prepare a standard sample of erythromycin with a concentration of 0.001g / mL, and its potency is 920U / mL;

[0031] The preparation concentration is 0.01g / mL suspension of thioerythromycin bacteria residue, its original potency is 73.4U / mL, and the pH is adjusted to be 8;

[0032] Add pectinase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.5g / mL, and the enzyme activity is 5000U / mL; After digestion, the titer of thioerythromycin bacteria residue was 3.67U / mL, and the titer of thioerythromycin bacteria residue was reduced by 95%.

Embodiment 2

[0034] Prepare a standard sample of erythromycin with a concentration of 0.003g / mL, and its potency is 2760U / mL;

[0035] The preparation concentration is 0.02g / mL suspension of thioerythromycin bacteria residue, its original potency is 220.1U / mL, and the pH is adjusted to be 7;

[0036] Add pectinase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.6g / mL, and the enzyme activity is 6000U / mL; After digestion, the titer of thioerythromycin bacteria residue was 20.9U / mL, and the titer of thioerythromycin bacteria residue was reduced by 91.5%.

Embodiment 3

[0038] Prepare a standard sample of erythromycin with a concentration of 0.005g / mL, and its potency is 4600U / mL;

[0039] The preparation concentration is 0.03g / mL suspension of thioerythromycin bacteria residue, its original potency is 78.15U / mL, adjust pH to be 9;

[0040] Add pectinase suspension to the thioerythromycin residue suspension, the enzyme concentration is 0.6g / mL, and the enzyme activity is 6000U / mL; After digestion, the titer of thioerythromycin bacteria residue was 5.5U / mL, and the titer of thioerythromycin bacteria residue was reduced by 93%.

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PUM

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Abstract

The invention provides a method for processing antibiotic residue by the utilization of pectase. pectase is used for processing antibiotic residue. The antibiotic residue is erythromycin sulfur residue. Erythromycin sulfur residue is processed by the use of pectase for 4-6 h under the following conditions: the temperature is 30-55 DEG C, pH is 7.0-9.5, and enzyme concentration is 0.5-0.65 g / mL. Titer of the erythromycin sulfur residue is reduced by 90% and above. The method of the invention is simple and feasible, processing cost is low, and secondary pollution is not generated. The method is energy-saving and environment-friendly. By the method, most residual antibiotics in the antibiotic residue can be removed. The method is convenient for subsequent processing of antibiotic residue and provides a certain data basis for the technology of processing antibiotic residue by the use of enzyme.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a method for enzymatic hydrolysis of residual antibiotic residues of antibiotic bacteria, in particular to a method for enzymatic hydrolysis of residual antibiotic residues of antibiotic bacteria by using pectinase. Background technique [0002] my country is a major producer of antibiotics. A large amount of solid waste, namely antibiotic residue, will be produced in the fermentation production of antibiotics. The main components of antibiotic residues are: microbial mycelium that produces specific antibiotics, reagents added during the extraction of antibiotics, incompletely utilized medium, degradation products of the medium, metabolites produced during fermentation, and unknown growth factors and small amounts of residual antibiotics. During the open-air stacking of antibiotic residues, on the one hand, it will cause air pollution and directly affect the ...

Claims

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Application Information

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IPC IPC(8): A23K10/14C05F5/00
CPCC05F5/008Y02A40/20
Inventor 臧立华姬丹丹李肖玲
Owner QILU UNIV OF TECH
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