Free fatty acid detection kit

Abstract

The invention relates to the technical field of biology, and discloses a free fatty acid detection kit. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from triphosadenine, metal ions, fatty acyl-CoA synthetase and 4-aminoantipyrine; the reagent R2 is prepared from coenzyme A, Trinder's chromogen and diacylglycerol transferase. According to the embodiment, metal ions are added into the reagent R1, the metal ions and sulfydryl on the coenzyme A which is the free coenzyme A obtained after a reaction with free fatty acid can have an ion exchange and complexing reaction to form a stable chelate, and the influence generated on hydrogen peroxide by sulfydryl on the coenzyme A is eliminated. Accordingly, the purposes of improving the accuracy of the free fatty acid detection kit and improving the stability of the kit are achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a free fatty acid detection kit. Background technique [0002] Free fatty acid, namely non-esterfied fatty acid (NEFA), is the product of hydrolysis of fat in the human body, reflecting the body's fat metabolism and blood lipid level. Symptoms of elevated levels are more common in obesity, diabetes, myocardial infarction, hyperthyroidism, acromegaly, severe liver disease, and starvation; symptoms of decreased levels can be seen in hypothyroidism, pituitary insufficiency, and Addison's sick. [0003] At present, the clinical detection of free fatty acids in human serum is mainly through free fatty acid detection kits, while free fatty acid detection kits sold in the market usually use enzymatic determination, and the main reaction principle of free fatty acid kits is mostly based on Trinder The reaction mode, the specific reaction process is as follows: [0004] Free fatty acid+ATP...

AI Technical Summary

Problems solved by technology

However, the kits for the enzymatic determination of free fatty acids have the following problems: the generated H 2 o 2 It will react rapidly with the sulfhydryl (-SH) structure on coenzyme A in the R2 reagent and consume part of the H 2 o 2 , which leads to H 2 o 2 Under the catalysis of peroxidase, it reacts with 4-aminoantipyrine and TOPS to reduce the red quinone imine compound, and the amount of red quinone imine compound is directly proportional to the content of free fatty acid in the sample. By measuring the red The concentration of free fatty acids in the sample can be obtained from the absorbance of the quinone imine compound, and the concentration of free fatty acids measured therefrom will also decrease accordingly, which may cause false negative results and cause errors in the judgment of the disease.

In order to solve the above problems, some manufacturers choose to add N-ethylmaleimide to the kit, but the reagent itself is unstable and will react with fatty acyl-CoA oxidase, resulting in acyl-CoA oxidase in the Trinder reaction. The amount of coenzyme A oxidase is difficult to control, resulting in errors in test results

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