Primer, probe, kit and method for detecting bacillus erysipelatos-suis by fluorescent quantitative PCR (Polymerase Chain Reaction)

Abstract

The invention discloses a primer, a probe, a kit and a method for detecting bacillus erysipelatos-suis by a fluorescent quantitative PCR (Polymerase Chain Reaction). The primer is shown as SEQ ID NO: 1 and SEQ ID NO: 2; the probe is shown as SEQ ID NO: 3. The method disclosed by the invention takes DNA (Deoxyribonucleic Acid) of a sample to be detected as a template for carrying out a fluorescent quantitative PCR amplification reaction; fluorescent signals are collected to draw a curve. The primer and the probe for detecting the bacillus erysipelatos-suis have very high specificity and sensitivity, can be used for detecting the bacillus erysipelatos-suis and can also be used for detecting various types of clinical samples, so that a clinical bacillus erysipelatos-suis infection condition can be rapidly monitored; the operation is simple and practical.

Description

technical field [0001] The invention belongs to the technical field of animal virology and molecular biology, and more specifically, the invention relates to a primer, a probe, a kit and a method for detecting Erysipelas suis by fluorescence quantitative PCR. Background technique [0002] Erysipelas suis is an acute, febrile infectious disease caused by Erysipelas suis bacillus, its main features are high fever, acute sepsis, skin rash (subacute), chronic verrucous endocarditis, skin necrosis and multiple non-suppurative Arthritis. This disease was once known as one of the "three major infectious diseases" in my country. In recent years, with the intensive development of pig raising, porcine erysipelas has gradually faded out of people's vision, but the disease has not been completely purified, and it has always occurred in various regions of the country. Occur sporadically, causing serious economic losses to pig farmers. [0003] Porcine erysipelas attenuated vaccine strai...

AI Technical Summary

Problems solved by technology

[0005] The main disadvantage of the viable culture count method is that it takes a long time to detect, and the same vaccine has two antigens with similar nutritional requirements, which will interfere with each other

The length of the test is due to the need for bacterial culture, which takes 24 to 48 hours; after the vaccine is diluted and inoculated on the plate medium, the inoculation position cannot be determined, and the two colonies overlap or cover each other, so the accurate count cannot be counted.

Additionally, bacterial cultures are potentially dangerous

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