Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

OSCC (oral squamous cell carcinoma) biomarker and application thereof

A technology for squamous cell carcinoma and oral cavity, which is applied in the application field of XIRP2, can solve the problems that tumors have not made major breakthroughs, etc.

Active Publication Date: 2017-02-22
BEIJING MEDINTELL BIOMED CO LTD
View PDF3 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no major breakthroughs have been made in the mechanism and treatment of tumors. How to detect tumor lesions early and cure them is a problem and opportunity for modern oncologists

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • OSCC (oral squamous cell carcinoma) biomarker and application thereof
  • OSCC (oral squamous cell carcinoma) biomarker and application thereof
  • OSCC (oral squamous cell carcinoma) biomarker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Screening for Gene Markers Related to Oral Squamous Cell Carcinoma

[0059] 1. Sample collection

[0060] 6 cases of surrounding normal mucosa tissue and oral squamous cell carcinoma tissue were collected, all of which were confirmed by pathological diagnosis. All patients did not receive any form of treatment before operation. The surgically resected samples were frozen in liquid nitrogen, and the patients gave informed consent, and all the above-mentioned samples were obtained with the consent of the organizational ethics committee.

[0061] 2. Preparation of RNA samples (operated using QIAGEN tissue RNA extraction kit)

[0062] Take out the tissue samples frozen in liquid nitrogen, put the tissue samples into a pre-cooled mortar for grinding, and extract and isolate RNA according to the instructions in the kit. details as follows:

[0063] 1) Add Trizol and place at room temperature for 5 minutes;

[0064] 2) Add 0.2ml of chloroform, vibrate the centrif...

Embodiment 2

[0077] Example 2 QPCR sequencing to verify the differential expression of XIRP2 gene

[0078] 1. Large-sample QPCR verification of differential expression of XIRP2 gene. According to the sample collection method in Example 1, 80 cases of normal mucosal tissues and 80 cases of oral squamous cell carcinoma tissues were selected.

[0079] 2. The RNA extraction steps are as described in Example 1.

[0080] 3. Reverse transcription:

[0081] 1) Reaction system:

[0082] Table 1 Reverse transcription reaction system

[0083]

[0084] 2) Reverse transcription reaction conditions

[0085] According to the reverse transcription reaction conditions in RNA PCR Kit (AMV) Ver.3.0.

[0086] 60min at 42°C, 2min at 99°C, 5min at 5°C.

[0087] 3) Polymerase chain reaction

[0088] 1) Primer design

[0089] QPCR amplification primers were designed according to the coding sequences of XIRP2 gene and GAPDH gene in Genebank, and synthesized by Biomed Biotech. The specific primer sequen...

Embodiment 3

[0104] Example 3 Differential expression of XIRP2 gene in oral squamous cell carcinoma cell lines

[0105] 1. Cell culture

[0106] Oral squamous cell carcinoma cell lines Tca8113 and HN13, and normal mucosal epithelial cell line HIOEC were purchased from the Ninth People's Hospital Affiliated to Shanghai Jiaotong University. The medium of HIOEC is K-SFM; the medium of Tca8113 and HN13 is DMEM; the culture medium containing 10% fetal bovine serum and 1% P / S was incubated at 37°C and 5% CO 2 , Cultivated in an incubator with a relative humidity of 90%. Change the medium once every 2-3 days, and use 0.25% EDTA-containing trypsin for routine digestion and passage.

[0107] 2. Extraction of total cellular RNA

[0108] 1) Stop the culture when the cells reach 80-90% confluency, digest with 0.25% trypsin and collect the cells in 1.5ml EP tubes, add lm1Trizol to each tube and shake slowly to break the cells, place on ice for 10min.

[0109] 2) Remove protein and DNA: add 0.2ml ch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an OSCC (oral squamous cell carcinoma) biomarker and an application thereof. The biomarker is XIRP2, an experiment proves that an XIRP2 gene expresses up regulation in OSCC tissue, and the silent XIRP2 gene can reduce transcription and translation of the gene to inhibit excessive proliferation of OSCC cells. The invention provides the application of the gene or an expression product of the gene in diagnosis and treatment of OSCC.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a biomarker in oral squamous cell carcinoma and its application, in particular to the application of XIRP2 which is significantly up-regulated in oral squamous cell carcinoma. Background technique [0002] Malignant tumors of the oral cavity are a class of diseases that seriously threaten human health. Oral squamous cell carcinoma (OSCC) accounts for about 90% of oral cancers. Like the occurrence of other tumors, the occurrence and development of OSCC is a long-term complex process with multiple genes, multiple molecules in a network structure, and multi-step and multi-stage interactions. The synergistic interaction between various tumor genes and molecules promotes the formation and development of tumor cells. At present, no major breakthrough has been made in the pathogenesis and treatment of tumors. How to detect tumor lesions early and cure them is a problem and an opportunity for m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N33/68G01N33/574A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/158G01N33/57407G01N33/57488G01N33/68G01N2333/47
Inventor 宋宏涛马翠任静
Owner BEIJING MEDINTELL BIOMED CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com