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CircRNA markers for early diagnosis of lung adenocarcinoma and application thereof

An early diagnosis and marker technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial detection/testing, etc., can solve the problems that circRNA has not been reported in lung cancer, and achieve broad clinical application prospects, strong specificity, and sensitivity sex high effect

Active Publication Date: 2017-02-22
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, circRNAs with early diagnostic value have been found in malignant tumors such as esophageal cancer, gastric cancer, and liver cancer, but there are no reports of related circRNAs in the field of lung cancer.

Method used

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  • CircRNA markers for early diagnosis of lung adenocarcinoma and application thereof
  • CircRNA markers for early diagnosis of lung adenocarcinoma and application thereof
  • CircRNA markers for early diagnosis of lung adenocarcinoma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1 Clinical sample collection:

[0022] Sixty pairs of paraffin tissue specimens of early (stage IA) lung adenocarcinoma and their paired normal lung tissues were collected from Zhongda Hospital Affiliated to Southeast University during 2013-2014. All patients signed an informed consent to participate in the scientific research.

[0023] 2 circRNA quantitative PCR experiment:

[0024] 2.1 RNA extraction and quantitative PCR

[0025] Total RNA was extracted from tissues using Trizol, and RNA was quantified using a microvolume spectrophotometer.

[0026] 2.2 Primers

[0027] A set of circRNAs provided by the present invention includes 8 circRNAs including circRNA_100323, circRNA_69774, circRNA_403738, circRNA_404449, circRNA_104855, circRNA_100983, circRNA_1011969, and circRNA_006718. They were all selected from the results of circRNA chip detection in patients with early lung adenocarcinoma, with GAPDH as the internal reference gene (SEQ ID No: 27), and the primer seq...

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Abstract

The invention belongs to the technical field of biology and discloses a group of circRNA markers for early diagnosis of lung adenocarcinoma and application thereof. The circRNA marker disclosed by the invention comprises four kinds of circRNA, namely circRNA-100323, circRNA-69774, circRNA-403738 and circRNA-404449. The invention has the beneficial effects that a group of novel molecular bio-markers with diagnostic values on lung adenocarcinoma are provided. According to verifications of clinical sample cases, the group of circRNA markers have high diagnostic specificity and sensitivity on early diagnosis of lung adenocarcinoma.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, and relates to a circRNA marker for early diagnosis of lung adenocarcinoma, related kits and applications thereof. Background technique [0002] Lung cancer is the first malignant tumor that seriously endangers human health, and its incidence rate (1.2 million / year) and mortality rate (1.1 million / year) both rank first in the world. The 2014 "Annual Report on Cancer Registration in my country" shows that lung cancer is also the malignant tumor with the highest morbidity and mortality in China (more than 600,000 new lung cancer patients are diagnosed each year in my country). The 5-year survival rate of lung cancer is only about 15.6%. The main reason is that most patients are diagnosed at an advanced stage, so early detection and early diagnosis of lung cancer are very important. [0003] Circular RNAs are a class of end-to-end non-coding RNAs produced by special alternative splicin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 朱晓莉王西勇张洪明韩淑华陈燕陈阳
Owner SOUTHEAST UNIV
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