Nostoc sphaeroides heterocyst embedded suspension breeding method

Abstract

The invention relates to a nostoc sphaeroides heterocyst embedded suspension breeding method. The method comprises the following steps: 1) inducing nostoc sphaeroides in a logarithmic phase by virtue of a nutrient solution, so that a multi-heterocyst series algae body is formed; 2) splitting the multi-heterocyst series algae body, and conducting separation so as to obtain individual nostoc sphaeroides heterocyst; and 3) cultivating the individual nostoc sphaeroides heterocyst, so that a nostoc sphaeroides algae body is obtained. According to the breeding method provided by the invention, on the basis of a firm bridging action which is achieved by virtue of agar powder and dried nostoc sphaeroides polysaccharide powder, the buoyancy of the nutrient solution is increased and the heterocyst is balanced in gravity and buoyancy, so that culture modes that nutrient substances are uniformly dispersed on one hand the heterocyst is uniformly dispersed and quietly suspends in the nutrient solution on the other hand are achieved; the breeding method is free from a pollution source and is high in success rate; and aerobic culture can be conducted without the consumption of electric energy, so that breeding cost is further reduced.

Description

technical field [0001] The invention relates to a cultivation technology of cyanobacteria, in particular to a method for mosaic suspension breeding of Gexianmi heteromorphic cells. Background technique [0002] Gexianmi is the common name of Nostoc sphaeoides Kützing in Nostoc Vauch, which belongs to Cyanophyta, Cyanophyceae, Nostocales, Nostocaceae ). Even more precious and rare than Fa Cai, Ge Xian Mi is also called green bird's nest. Not only that, Gexianmi contains 18 kinds of amino acids, including eight kinds of amino acids necessary for the human body. The total dry matter protein is as high as about 56%, the crude fat is 8.11%, the carbohydrate is 12.69%, the ash content is 10.88%, and the chlorophyll is 30.98mg / g. Ascorbic acid 5.50mg / g, vitamin C content close to fresh jujube, more than 5 times higher than hawthorn, 5 times higher than citrus; vitamin B1, B2 higher than general bacteria and algae; rich in minerals and trace elements, as well as phycobiliprotein ...

AI Technical Summary

Problems solved by technology

The liquid inflated suspension culture used in the prior art, the dynamic suspension of the algae breeding section consumes more electric energy, which is not conducive to the control of production costs, and the ventilation will bring pollution sources in the air, and it is easy to be contaminated with miscellaneous algae, bacteria, aquatic animals, dust, and mosquito larvae This leads to a high failure rate of breeding, if the air is treated, the cost of breeding will be increased

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