Preparation method of phosphate esterification bletilla striata polysaccharide

A technology of bletilla striata polysaccharides and phosphate esters, applied in the field of medicinal chemistry, can solve problems such as high cross-linking degree, low biological activity, and poor solubility, and achieve the effects of simple operation, good solubility, and strong antioxidant properties

Active Publication Date: 2017-02-22
SHANGHAI INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the invention provides a kind of preparation method of phosphated bletilla striata polysaccharide, the preparation method of described this phosphated bletilla striata polysaccharide should solve the phosphorylated polysaccharide prepared in the prior art The technical problems of high cross-linking degree, poor solubility and low biological activity

Method used

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  • Preparation method of phosphate esterification bletilla striata polysaccharide
  • Preparation method of phosphate esterification bletilla striata polysaccharide
  • Preparation method of phosphate esterification bletilla striata polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Weigh 1 g of Bletilla striata polysaccharide and dissolve it in 20 mL of distilled water, and stir until the dissolution is complete. Heat up, and when the temperature reaches 90℃, add 10ml 0.05g / ml sodium polyphosphate solution, 25ml 5g / L sodium sulfate solution into the reaction vessel, adjust pH=4 with 1mol / L HCl, stir in water bath and react for 4hr , Then add 3 times the volume of absolute ethanol, alcohol precipitation overnight, vacuum suction filtration, dissolve the product with a small amount of water, place it in a dialysis bag for 24hr dialysis, and freeze-dry to obtain Bletilla striata polysaccharide phosphate with a degree of substitution of 0.036.

Embodiment 2

[0024] Weigh 1 g of Bletilla striata polysaccharide and dissolve it in 20 mL of distilled water, and stir until the dissolution is complete. Heat up, and when the temperature reaches 80℃, add 20ml of 0.05g / ml sodium polyphosphate solution and 25ml of 5g / L sodium sulfate solution into the reaction vessel in turn, adjust pH=5 with 1mol / L HCl, stir in water bath, and react for 5hr , Then add 3 times the volume of absolute ethanol, alcohol precipitation overnight, vacuum suction filtration, dissolve the product with a small amount of water, place it in a dialysis bag and dialyze for 24 hours, freeze-dry to obtain Bletilla striata polysaccharide phosphate, the degree of substitution is 0.042.

Embodiment 3

[0026] Weigh 1 g of Bletilla striata polysaccharide and dissolve it in 20 mL of distilled water, and stir until the dissolution is complete. Heat up, and when the temperature reaches 90℃, add 15ml of 0.05g / ml sodium polyphosphate solution and 25ml of 5g / L sodium sulfate solution into the reaction vessel, adjust pH=6 with 1mol / L HCl, stir in water bath, and react for 6hr , Then add 3 times the volume of absolute ethanol, alcohol precipitation overnight, vacuum filtration, dissolve the product with a small amount of water, place it in a dialysis bag for 24hr dialysis, and freeze-dry to obtain Bletilla striata polysaccharide phosphate with a degree of substitution of 0.041.

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Abstract

The invention provides a preparation method of phosphate esterification bletilla striata polysaccharide. The method includes the steps: weighing dry bletilla striata polysaccharide samples, preparing into solution, and sequentially adding sodium polyphosphate and sodium sulfate; adjusting a pH (potential of hydrogen) value of a reaction system, performing reaction for 3-6hr at the temperature ranging from 70 DEG C to 95 DEG C, then performing alcohol precipitation, dialyzing, and drying to obtain the phosphate esterification bletilla striata polysaccharide. The concentration of the solution ranges from 1% to 10%, the weight ratio of the bletilla striata polysaccharide, the sodium polyphosphate and the sodium sulfate is (5-20): (1-10):1, and the pH value of the reaction system ranges from 4 to 6. The phosphate esterification bletilla striata polysaccharide obtained by the preparation method is medium in substitution degree, good in dissolution performance and simple to operate and solves the problem of poor solubility caused by synthesis under the alkaline conditions.

Description

Technical field [0001] The present invention belongs to the field of medicinal chemistry, and relates to a Bletilla striata polysaccharide, specifically a preparation method of a phosphated Bletilla striata polysaccharide. Background technique [0002] Bletilla striata belongs to the genus Bletilla of the orchid family, a perennial herb. Also known as Baiji, Zilan, Zihui, Baili, etc., it has the effects of astringent and hemostasis, swelling and muscle growth, clearing heat and dampness, and has been used as a medicinal material for nearly a thousand years. The bulb tissue of Bletilla striata is rich in natural water-soluble polysaccharides. [0003] The activity of polysaccharides is directly or indirectly restricted by its structure. Through molecular modification, polysaccharide derivatives with higher biological activity and more versatile applications can be obtained. Modification methods of polysaccharide molecules include chemical, biological and physical methods. Phospho...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/00
Inventor 俞苓王益莉顾飞燕黄怡雯蔡梦婷
Owner SHANGHAI INSTITUTE OF TECHNOLOGY
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