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Preparation method and application of chitosan-microsphere immunoaffinity adsorbent

A technology of chitosan microspheres and immunophiles, applied in chemical instruments and methods, testing dairy products, alkali metal oxides/hydroxides, etc., can solve the problems of uneven size of microspheres, restrictions on wide use, instability, etc. problems, to achieve the effect of wide pH stability, uniform particle size, and abundant sources

Inactive Publication Date: 2017-02-22
JIANGSU UNIV
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Problems solved by technology

At present, chitosan microspheres prepared as carrier chromatography fillers have disadvantages such as uneven size of microspheres and instability under acidic conditions, which limit their wide application.

Method used

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  • Preparation method and application of chitosan-microsphere immunoaffinity adsorbent
  • Preparation method and application of chitosan-microsphere immunoaffinity adsorbent
  • Preparation method and application of chitosan-microsphere immunoaffinity adsorbent

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Embodiment 1

[0025] Embodiment 1 A kind of pH stable, the preparation of uniform particle size chitosan microsphere immunoaffinity adsorbent

[0026] (1) Preparation of chitosan microspheres with stable pH and uniform particle size:

[0027] A. get 0.24g chitosan, dissolve in 12mL 2% acetic acid solution and be made into 2% chitosan solution;

[0028] b. Add 98mL of paraffin oil containing 2% span 80, emulsify for 3h;

[0029] c. Add 3.6 mL of cross-linking agent for cross-linking reaction for 3 hours, and add sodium hydroxide solution to keep the pH of the system at 9-10;

[0030] d. After the reaction is completed, centrifuge at 3000rpm for 8min to collect the microspheres, then extract them with petroleum ether for 6h, ethanol gradient elution, wash with distilled water until neutral and use 5M NaBH 4 Restored overnight, and finally washed with distilled water until neutral, freeze-dried for later use.

[0031] (2) Preparation of immunoaffinity adsorbent, the present embodiment takes...

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Abstract

The invention provides a preparation method and application of a chitosan-microsphere immunoaffinity adsorbent and belongs to the technical fields of development and application of natural polysaccharide and preparation of functional materials. The preparation method comprises the following steps of: preparing chitosan solution, weighing and taking liquid paraffin containing an emulsifying agent span 80, adding the liquid paraffin into the chitosan solution, stirring, and emulsifying to obtain mixed liquid; adding a crosslinking agent to carry out stirring crosslinking, and carrying out Soxhlet extraction, alcohol rinsing, washing, reduction and rewashing to obtain chitosan microspheres; stirring and coupling the chitosan microspheres and antibiotics, washing with distilled water and PBS (Phosphatic Buffer Solution), and finally obtaining the chitosan-microsphere immunoaffinity adsorbent. The chitosan-microsphere immunoaffinity adsorbent prepared by the invention is homogeneous in particle size of the microspheres, has better pH stability and can be used for purifying, separating and measuring the antibiotics in a food sample.

Description

technical field [0001] The invention relates to the preparation and application of a chitosan microsphere immunoaffinity adsorbent with stable pH and uniform particle size, and belongs to the technical field of development and application of natural polysaccharides and preparation of functional materials. Background technique [0002] Antibiotics have important applications in clinical treatment and animal husbandry production, but long-term and excessive use has brought serious harm to the ecological environment and human health. On the one hand, the emergence of drug resistance of pathogenic microorganisms; residues in the environment. Many countries and international organizations, including my country, have clearly stipulated that the use of antibiotics in animal husbandry is prohibited or limited, and relevant monitoring work has begun. At present, HPLC, GC / MS or LC / MS are the main analytical methods for the detection of antibiotic residues in agricultural products, an...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/28B01J20/281B01J20/30G01N33/577G01N33/04
CPCB01J20/24B01J20/28019B01J20/281G01N33/04G01N33/577
Inventor 王云杨雅梅王娅丹牛瑞朱新生
Owner JIANGSU UNIV
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