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Marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment

A technology of marine bacteria and orchids, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of weakening resistance, polluting the environment, endangering human health, etc., and achieve the effect of preventing and protecting the environment

Active Publication Date: 2017-02-15
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main strategies to control stem rot are chemical control and cultivating disease-resistant varieties. Chemical control pollutes the environment and endangers human health. It is difficult to breed resistant varieties and there is a risk of weakening resistance. Therefore, biological control is because of its safety, high efficiency, and Cruelty-free is the most preferred method
In particular, there have been many successful examples of using marine microorganisms to control plant diseases, but there have been no reports at home and abroad on the use of marine microorganisms to control orchid stem rot

Method used

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  • Marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment
  • Marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment
  • Marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening and Identification of Marine Bacteria

[0023] 1. Screening of marine bacteria

[0024] The bacterial strain of the present invention is obtained by separating and obtaining from marine fish in Haikou City, Hainan, and the specific method is as follows: taking different parts such as viscera, intestines, gills and fins of the marine fish to separate and screen the marine bacteria. The plate dilution method is used to separate the symbiotic bacteria of marine fish, take an appropriate amount of samples, and wash them repeatedly with sterile seawater to remove the non-symbiotic microorganisms on the surface of the samples as much as possible, and then use sterile absorbent paper to dry the surface of the samples. Wipe with 75% ethanol, then weigh the sample, homogenize, take the supernatant, and use sterile sea water according to 10 -8 、10 -9 、10 -10 、10 -11 、10 -12 Dilute, draw 200 μL of liquid, spread it evenly on the agar medium (NA) medium pl...

Embodiment 2

[0030] Embodiment 2: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Biological activity of BA-3 culture filtrate against orchid stem rot

[0031] 1. Inhibitory effect of BA-3 culture filtrate on spore germination of orchid stem rot fungus

[0032]Using the glass slide spore germination method, after culturing the spore suspension of the orchid stem rot fungus for 12 h, the optical

[0033] Observed under the microscope, in the control without BA-3 culture medium, about 98% of the spores germinated and formed slender and uniform germ tubes, but in the culture filtrate 10 times, 40 times, The spores in 100 times only have a germination rate of 1-40%, and the germ tubes are short, and some germ tubes are deformed and uneven. The specific germination rate and inhibition rate are shown in Table 2.

[0034] Table 2 Effect of culture filtrate of marine bacteria BA-3 on germination of orchid stem rot spores

[0035]

[0036] 2. Effects of different concentrations of...

Embodiment 3

[0048] Embodiment 3, bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Determination of the control effect of BA-3 culture filtrate on orchid stem rot

[0049] 1. Determination of the control effect of the culture filtrate of strain BA-3 on stem rot pathogen: the obtained culture filtrate was diluted with sterile water to prepare solutions with concentrations of 200 mg / L, 400 mg / L and 800 mg / L. The orchid stem rot fungus was cultivated with PDA medium, and then made into 10 8 The cfu / mL spore suspension is ready for use. Three inoculation treatments were set up: treatment I was inoculated with Bacillus amyloliquefaciens BA-3 culture solution and stem rot fungus spore suspension at the same time; treatment II was inoculated with Bacillus amyloliquefaciens culture solution first, and then inoculated stem rot fungus spore suspension 2 days later; Treatment Ⅲ was inoculated with the spore suspension of the stem rot fungus first, and then inoculated with the culture solu...

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Abstract

The invention relates to marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment, and belongs to the technical field of microorganisms. The preservation number for the marine bacteria BA-3 is CGMCC No.12745. The marine bacteria BA-3 capable of preventing and treating plant diseases, caused by stalk rot pathogens and root rot pathogens, of orchids can grow together with the orchids especially, can survive stably in orchid plants and can prevent and treat stalk rot diseases or root rot diseases of the orchids effectively.

Description

technical field [0001] The invention relates to a strain of marine bacteria BA-3 and its application in preventing orchid diseases, and belongs to the field of microbial prevention and control. Background technique [0002] At present, the prevention and control of plant diseases is mainly based on chemical control. However, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and resistance of pathogenic bacteria. Biocontrol bacteria are characterized by their low toxicity and no pollution. , not easy to produce drug resistance and easy to get raw materials and other characteristics are recognized as ideal microorganisms. Microorganisms used to control plant diseases are mainly fungi and bacteria. The mechanism of biocontrol bacteria mainly includes three aspects. The first is antagonism, which inhibits the growth of pathogenic bacteria through the production of antibiotic substances; th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 姚锦爱余德亿兰成忠黄俊义黄鹏
Owner INST OF PLANT PROTECTION FAAS
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