Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof
A technology of nitrogen-fixing microorganisms and bacterial fertilizers, which is applied in the preparation of organic fertilizers, fertilizers made from biological waste, nitrogen fertilizers, etc., can solve the problems of poor growth and development, limited ability to adapt to the environment, and difficulty in commercialization, etc., and achieve a wide range of PH adaptability sex, stimulating plant growth, and preventing spore germination
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Embodiment 1
[0032] A compound nitrogen-fixing microbial fertilizer, characterized in that the raw material ratio of the compound nitrogen-fixing microbial fertilizer is: 20 parts of Bacillus flexus fermentation broth, 50 parts of Clostridium pasteurianus fermentation broth, and Klebsiella indica 30 parts of fermented liquid.
[0033] A kind of compound nitrogen-fixing microbial bacterial fertilizer according to claim 1, is characterized in that, its preparation method comprises the following steps:
[0034] Step 1, the preparation of Bacillus flexus fermented liquid
[0035] Take out the preservation tube of Bacillus flexus, use nitrogen-free solid medium to draw plates for recovery, and culture at 30°C for 96 hours. Pick a single colony under the plate and inoculate it into a nitrogen-free solid medium, culture it in a 30°C incubator for 72 hours, wash the bacterial lawn in three eggplant bottles with 3000ml sterile water, and inoculate it into a 300L curved In the 500L fermentation ta...
Embodiment 2
[0051] The application effect of the composite nitrogen-fixing microbial bacterial fertilizer prepared in Example 1 on pepper (abbreviated bacterial fertilizer)
[0052] 1 Test time: May-August 2015
[0053] 2. Test location: Zhongqiao Village, Tantou Town, Longhui County, Hunan Province
[0054] 3. experiment method:
[0055] Test group: When transplanting, dip the seedlings with 200 times of bacterial fertilizer dilution for 30 minutes and plant them. After 10 days after planting, use 1000 times of bacterial fertilizer dilution to irrigate the roots once. The total bacteria used is 2 kg / mu, and the total nitrogen fertilizer is 20 kg / mu; the control group dipped and irrigated the roots with clear water, and the total nitrogen fertilizer application was 40 kg / mu;
[0056] 4. test results:
[0057] , The impact on output and economic benefits The results found that the average yield of the compound nitrogen-fixing microbial fertilizer was 726 kg / mu, and the average yiel...
Embodiment 3
[0062] Application effect of compound nitrogen-fixing microbial fertilizer on cucumber
[0063] 1. Test location: a grower in Shouguang City, Shandong Province
[0064] 2. Test method: Test group: When transplanting, dip the seedlings with a 200-fold dilution of bacterial fertilizer for 20 minutes to plant, and 10 days after planting, irrigate the roots once with a 1000-fold dilution of bacterial fertilizer, and the total amount of bacterial fertilizer applied is 2 kg. Nitrogen fertilizer is 15 kilograms; Control group: dip root with clear water and irrigate root, and total nitrogen fertilizer is 30 kilograms / mu;
[0065]3. Test results: After transplanting, it was found that the leaves of the plants with roots were fresher, and the growth potential was good, and there was no wilting. The plants using the compound nitrogen-fixing microbial fertilizer showed flowers one week earlier than the control group. After most of the flowers have bloomed, it is found that the flowers...
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