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Bud breeding method of bud organ tissue culture of eucalyptol type Cinnamomum longepaniculatum

A technology of eucalyptol and oil camphor, which is applied in the field of plant asexual reproduction, can solve the problems of low bud proliferation coefficient, browning of first-generation buds, and slow growth, and achieve the effects of rapid growth, inhibition of brown death, and lush branches and leaves

Inactive Publication Date: 2017-02-15
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems such as the browning or vitrification of the first-generation buds in the eucalyptol-type oil camphor tissue culture process, the bud proliferation coefficient is low, and the growth is slow. Fast eucalyptol-type oil camphor bud organ tissue culture bud propagation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In forest stands, green belts or scattered camphor trees, select individual plants with straight and round stems, lush branches and leaves, fast growth, and strong camphor smell in the leaves, and take the individual leaves back to the room for eucalyptol quantification. Qualitative analysis showed that the leaves with a leaf oil yield of more than 1.5% and a eucalyptol content of more than 70% were identified as eucalyptol-type camphor explant collection plants. In a sunny climate for at least 3 consecutive days, the year-old shoots that grow vigorously and have no damage by diseases and insect pests on the periphery of the canopy of the eucalyptol type camphor tree are collected as explants.

[0029]Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, soak the explants in 0.2% sodium hypochlorite solution for 10 minutes, rinse the explants with pure water for 4-5 times, and then wash the explants in volume concentration Soak in 0....

Embodiment 2

[0035] In forest stands, green belts or scattered camphor trees, select individual plants with straight and round stems, lush branches and leaves, fast growth, and strong camphor smell in the leaves, and take the individual leaves back to the room for eucalyptol quantification. Qualitative analysis showed that the leaves with a leaf oil yield of more than 1.5% and a eucalyptol content of more than 70% were identified as eucalyptol-type camphor explant collection plants. In a sunny climate for at least 3 consecutive days, the year-old shoots that grow vigorously and have no damage by diseases and insect pests on the periphery of the canopy of the eucalyptol type camphor tree are collected as explants.

[0036] Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, soak the explants in 0.2% sodium hypochlorite solution for 10 minutes, rinse the explants with pure water for 4-5 times, and then wash the explants in volume concentration Soak in 0...

Embodiment 3

[0042] In forest stands, green belts or scattered camphor trees, select individual plants with straight and round stems, lush branches and leaves, fast growth, and strong camphor smell in the leaves, and take the individual leaves back to the room for eucalyptol quantification. Qualitative analysis showed that the leaves with a leaf oil yield of more than 1.5% and a eucalyptol content of more than 70% were identified as eucalyptol-type camphor explant collection plants. In a sunny climate for at least 3 consecutive days, the year-old shoots that grow vigorously and have no damage by diseases and insect pests on the periphery of the canopy of the eucalyptol type camphor tree are collected as explants.

[0043] Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, soak the explants in 0.2% sodium hypochlorite solution for 15 minutes, rinse the explants with pure water 4-5 times, and then wash the explants in volume concentration Soak in 0.1% ...

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PUM

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Abstract

The invention discloses a bud breeding method of bud organ tissue culture of eucalyptol type Cinnamomum longepaniculatum. The method comprises explant selection, explant processing, primary mud induction culture, propagation culture and bud strengthening culture, and concretely comprises the following steps: collecting annual tender branches of eucalyptol type Cinnamomum longepaniculatum trees with robustly grown crown periphery and without pest or disease damages as explants, carrying out trimming, sterilizing and disinfecting treatment on the explants, inoculating a primary bud induction medium with the treated explants to obtain primary buds, inoculating a propagation medium with the primary buds, carrying out propagation culture for 5-6 cycles to form adventitious buds, inoculating a bud strengthening medium with the adventitious buds, and culturing the adventitious buds to form a large amount of strong single buds with the height being not lower than 2 cm. The method has the advantages of simple operating process, short culture cycle, large proliferation bud, good quality, realization of industrial technologic requirements of tissue culture seedlings with the propagation coefficient reaching 4 or above, and very good economic, ecologic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction, and relates to the tissue culture technology of camphor tree, in particular to a method for tissue culture and propagation of bud organs of eucalyptol type camphor tree. Background technique [0002] Eucalyptus oil (cineole, C 10 h 18 O) Plain oil camphor is a kind of chemical camphora (Cinnamomum camphora (L.) Presl), also known as camphor, which is a national second-class protected plant, mainly distributed in Jiangxi, Hunan and Guangdong in China. , Fujian, Hainan, Guangxi, Sichuan, Taiwan and other places, and it is also distributed in Vietnam, South Korea and Japan abroad. Grows in hills and low mountains. It is a multi-purpose excellent tree species that is used for timber, oil, and landscape gardening. Eucalyptol is a colorless, transparent, volatile oily liquid with a camphor smell and a spicy cool taste. It mainly exists in the volatile oils of aromatic plants such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 蔡玲陆顺忠谭桂菲黄金使黎素平覃玉凤
Owner GUANGXI FORESTRY RES INST
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