Rooting culture and hardening seedling method of cunninghamia lanceolata tissue-cultured seedling
A technology of rooting culture and rooting medium, applied in the field of agriculture, can solve the problems of uneven genetic quality, high production cost, and low transplanting survival rate of production seeds
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[0025] 6 Chinese fir clones were cultivated in the Guangdong Academy of Forestry Sciences, a total of 6,000 seedlings were rooted, and 5,000 seedlings were transplanted after hardening. The Chinese fir tissue culture seedling rooting culture that present embodiment adopts and the method for hardening seedling is specific as follows:
[0026] (1) Selection of tissue cultured shoots for rooting culture: On September 15, 2015, among the Chinese fir tissue cultured shoots for multiplication culture, the shoots with strong growth, dark green leaves and a length of more than 3 cm were selected for rooting to cultivate;
[0027] (2) Rooting inoculation: on the ultra-clean workbench, cut the part of the top tip of the tissue cultured shoots used for rooting culture that is 2.5 cm to 3.0 cm down, and insert it into the glass culture bottle that has prepared the rooting medium in time 12 to 15 seedlings were inoculated in each bottle, and a plastic sealing cap was added. The volume of ...
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