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Immune-lateral-chromatography detecting system and preparing method thereof

A technology of immune lateral chromatography and detection system, applied in the field of immune lateral chromatography detection system and its preparation, which can solve the problems of poor creeping effect, low assembly efficiency, and influence on precision, so as to improve system precision and repeatability, low assembly efficiency, and improved assembly efficiency

Active Publication Date: 2017-02-01
北京康思润业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current fluorescent immunological lateral flow chromatography method generally uses nitrocellulose membrane as the creeping material. However, the length of the nitrocellulose membrane used in the current detection device is long, and the creeping effect is not good. In this case, it is necessary to cut the nitrocellulose membrane, sample pad, and water-absorbent pad first (5mm wide) and then assemble them. The assembly efficiency is low and the process is cumbersome. The order of the sample pads is random, and the error in the position of the manual sample pad will affect the precision of the final test.

Method used

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  • Immune-lateral-chromatography detecting system and preparing method thereof
  • Immune-lateral-chromatography detecting system and preparing method thereof
  • Immune-lateral-chromatography detecting system and preparing method thereof

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Embodiment 1

[0043] The preparation method of the immune lateral flow detection system provided by the invention is as follows:

[0044] Paste the upper and lower rubber sheets:

[0045] Nitrocellulose membrane specification: 23*3.5cm,

[0046] Rubber sheet A specification: 23*(1.9+0.2)cm, there is a tangent line at 2mm on one side;

[0047] Rubber sheet B specification: 23*(1.6+0.2)cm, there is a tangent line at 2mm on one side;

[0048] Note: There is double-sided adhesive on the surface of the rubber plate, and the tangent line on both sides is to cut the double-sided adhesive, which is convenient for subsequent connection with the nitrocellulose membrane.

[0049] Tear off the 2mm wide double-sided adhesive outer stickers on the outside of the tangent line of the two rubber sheets, respectively, and stick the two rubber sheets on both sides of the nitrocellulose membrane, and the part that has not been torn off the double-sided adhesive is on the outside of the nitrocellulose membran...

Embodiment 2

[0071] A preparation method of an immunological lateral flow detection system, comprising the steps of:

[0072] Coating antibody: Dilute human myoglobin (MYO) detection antibody 1 with coating buffer to a fixed concentration (2.0mg / ml), and control reagent 1 (goat anti-chicken IgY) to a fixed concentration (2.0mg / ml) , using the special coating equipment XYZ3060 of BIODOT Company to coat the above two liquids on the Sartorius nitrocellulose membrane 140 (NC membrane), and dry in a 37°C drying oven for 4 hours (to obtain the detection band and the quality control band respectively) ,spare. Coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as protective agent.

[0073] Labeled antibody: human myoglobin (MYO) detection antibody 2 and control reagent 2 (chicken IgY) were fluorescently labeled with latex, and stored in storage solution (50mMol / l Tris, 0.5% BSA, pH7.8).

[0074] Marker preparation: Spray 5 microliters of the above-mentioned labeled antib...

Embodiment 3

[0083] Coating antibody: Dilute troponin (cTnI) detection antibody 1 with coating buffer to a concentration of 3.0 mg / ml, and control reagent 1 (goat anti-chicken IgY) to a concentration of 3.0 mg / ml, using special coating equipment The XYZ3060 of BIODOT company coated the above two liquids on the Sartorius nitrocellulose membrane (NC), dried in a 37°C oven for 4 hours, and set aside. Coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as protective agent.

[0084] Labeled antibodies: Troponin (cTnI) detection antibody 2 and control reagent 2 (chicken IgY) were fluorescently labeled with latex, stored in storage solution, and used for later use (50mM Tris, 0.5% BSA, pH 7.8).

[0085] Marker preparation: Spray 5 microliters of the above-mentioned labeled antibody into the inner wall of the tip according to the required concentration, and dry it for later use.

[0086] Sample pad preparation: Soak or spray the sample treatment solution in or on the sampl...

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Abstract

The invention discloses an immune-lateral-chromatography detecting system and a preparing method thereof. The detecting system comprises a bottom plate, a sample pad and a water absorption pad, wherein the bottom plate comprises a first rubber plate for bearing the sample pad, a nitrocellulose film and a second rubber plate for bearing the water absorption pad, the first rubber plate and the second rubber plate are fixedly connected with the two ends of the nitrocellulose film respectively, and a detecting belt and a quality control belt are arranged on the nitrocellulose film; the sample pad is fixed on the first rubber plate and is in contact with the nitrocellulose film to guarantee to climb water; the water absorption pad is fixed on the second rubber plate and is in contact with the nitrocellulose film to guarantee to climb water. The detecting system has the advantages of being low in cost and high in water climbing effect.

Description

technical field [0001] The invention relates to the medical field, in particular to an immunological lateral flow detection system and a preparation method thereof. Background technique [0002] Immunolateral flow-through diagnostic technology is a stable and practical technology suitable for use in various point-of-care tests (POCT) or on-site. According to the labeling part, it is mainly divided into colloidal gold immunological lateral flow chromatography, ordinary fluorescent immunological lateral flow chromatography, time resolved fluorescent Directional chromatography, etc., according to the coating part, there are mainly nitrocellulose membrane, composite material (fusion5), microfluidics, etc. [0003] With the development of immunochromatography (immunochromatography) and colloidal gold technology, especially after the 1990s, colloidal gold immunochromatography (Gold immunochromatography assay, GICA) has been widely used in in vitro diagnostic disease detection. H...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/543G01N33/558
Inventor 张军王龙
Owner 北京康思润业生物技术有限公司
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