Rapid detection quantum dot immunochromatographic test strip, kit and preparation method

An immunochromatographic test paper and quantum dot technology, which is applied in the field of clinical medical diagnosis, can solve the problems of long detection period, high cost of detection reagents, and unsuitability for rapid clinical diagnosis, and achieve short detection period, increased sensitivity and accuracy, and preparation short cycle effect

Inactive Publication Date: 2017-02-01
江苏莱博德医疗科技有限公司
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Problems solved by technology

[0007] To sum up, the POCT machine for platelet aggregation inhibition rate detection of clopidogrel resistance has the following disadvantages: professional detection equipment, high cost of a single detection reagent; enzyme-linked immunoassay kit detection has the following disadvantages: long detection cycle, not suitable for clinical rapid Diagnosis; therefore, there is an urgent need for detection reagents that can rapidly detect clopidogrel resistance in clinic

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  • Rapid detection quantum dot immunochromatographic test strip, kit and preparation method
  • Rapid detection quantum dot immunochromatographic test strip, kit and preparation method
  • Rapid detection quantum dot immunochromatographic test strip, kit and preparation method

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preparation example Construction

[0047] The preparation method of the quantum dot immunochromatography test strip for rapid detection of the curative effect of clopidogrel comprises the following steps:

[0048] Step 1) Preparation of probe immobilization pad 3: preparation of the conjugated quantum dots and phosphorylated VASP protein mouse-derived monoclonal antibody: quantum dots activated by the activator NHS, and a certain proportion of phosphorylated VASP protein mouse-derived Monoclonal Antibody Mix. The prepared conjugate was cross-linked under the action of the cross-linking agent EDC, and the conjugate was diluted to 1 μM with a diluent for use. Glass cellulose membrane pretreatment: Pretreatment with treatment liquid containing PEG20000, BSA, Tween-20 and Trion X-100, after drying, 1μM conjugates were evenly sprayed at a speed of 1-2μl / cm on a width of 1.0- On the 1.2 cm pretreated glass fiber membrane, the probe immobilization pad was prepared after drying at 37° C. for 24 h.

[0049] Step 2) De...

Embodiment 1

[0073] Quantum dot immunochromatographic detection of the curative effect of embodiment 1 clopidogrel

[0074] Step 1: Preparation of Probe Fixing Pad

[0075] Take the quantum dots in an ultrafiltration tube (30KD), centrifuge at 8000 rpm for 10 minutes, replace the quantum dot dilution with 0.1MMES buffer, adjust the concentration to 5μM, and set aside; take the phosphorylated VASP protein mouse monoclonal antibody in Ultrafiltration tube (30KD), 8000 rpm, centrifuge for 10 minutes, replace the antibody diluent with 0.1M MES buffer, adjust the concentration to 2mg / ml, and set aside.

[0076] Take 100 μl of quantum dots in a pyrogen-free centrifuge tube, add 10 μl of N-hydroxysuccinimide (NHS) with a concentration of 10 mM, mix well, let stand at room temperature, and activate the quantum dots for 15 minutes. Add 100 μl of 2 mg / ml phosphorylated VASP protein mouse monoclonal antibody, mix well, add 10 μl of 10 mM 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (...

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Abstract

The invention discloses a rapid detection quantum dot immunochromatographic test strip, a kit, a preparation method and a use method. Phosphorylated VASP protein murine monoclonal antibodies and quantum dot conjugates are sprayed onto glass fiber membranes to obtain a probe fixation mat, the VASP protein murine monoclonal antibodies are enveloped on a detection membrane detection line, goat-anti-mouse polyclonal antibodies are enveloped on a detection membrane quality control line, a detection membrane, the probe fixation mat, a sample mat and absorbent paper are sequentially assembled on a PVC (polyvinyl chloride) substrate, and the substrate is cut into small strips to obtain the test strip. The quantum dot immunochromatographic test strip is simple and convenient to operate, high in sensitivity and accuracy, low in cost and suitable for clinical rapid detection.

Description

technical field [0001] The invention relates to the technical field of clinical medical diagnosis, in particular to a quantum dot immunochromatographic test strip for rapidly detecting the curative effect of clopidogrel, a kit, a preparation method and a use method thereof. Background technique [0002] In the 21st century, there are more than 270 million patients with cardiovascular and cerebrovascular diseases in my country, and nearly 3 million people die of cardiovascular and cerebrovascular diseases every year, which brings heavy economic and social burdens to families and society. Cardiovascular and cerebrovascular diseases (such as coronary heart disease, myocardial infarction, and stroke) are all due to long-term atherosclerosis, which in turn causes abnormal platelet function, and finally develops in the form of thrombus or hemorrhage. The 2010 "Guidelines for the Prevention and Treatment of Hypertension in China" pointed out that aspirin or clopidogrel should be in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/558G01N33/577G01N33/58
CPCG01N33/588G01N33/533G01N33/558G01N33/577
Inventor 郝中禾
Owner 江苏莱博德医疗科技有限公司
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