Selective ultra-early fertilization observation and remedying method for preventing fertilization failure

Abstract

The present invention discloses a selective ultra-early fertilization observation and remedying method for preventing fertilization failure. The selective ultra-early fertilization observation and remedying method comprises that (1) early fertilization observation is performed at 40-60 min after fertilization; (2) at 3-6 layers of cumulus cells closely connected to oocytes, an annular fracture zone is presented or all cumulus cells are scattered; (3) when the light of a microscope passes through the fracture zone, an O-shaped light ring or completely-scattered condition is presented; and (4) the oocytes of the patient having the fertilization ability show the characteristics while the oocytes of the patient with the fertilization failure do not show the characteristics. According to the present invention, with the method, the subject with the fertilization failure can be effectively and accurately found as early as possible, and compared with the conventional method, the ICSI remedying has the following clinical results that the high quality embryo blastula developmental rate is high while the multi-sperm fertilization rate is not increased; and with the method, the fertilization failure can be found as early as possible and the ICSI remedying can be performed as early as possible, the sperm-egg co-incubation time can be reduced, the interference on most of the oocytes having the normal fertilization ability can be avoided, the embryonic development potential can be improved, the live birth rate can be improved, the labor time can be reduced, and the effectiveness of IVF can be improved.

Description

technical field [0001] The invention relates to a method for preventing fertilization failure, in particular to a selective ultra-early fertilization observation and remedy method for preventing fertilization failure. Background technique [0002] Currently, in conventional in vitro fertilization techniques (in vitro fertilization, IVF), the probability of fertilization failure is about 5%-10%. For patients who lost their chances of conception in this cycle, it is undoubtedly a huge pain. Therefore, how to avoid fertilization failure in IVF cycles is a research hotspot in reproductive medicine. [0003] In theory, the earlier salvage ICSI is performed, the better the pregnancy outcome of the patient. It has been confirmed that the optimal fertilization "window" of human oocytes is 39-40h after HCG. However, the current classic early fertilization observation-rescue ICSI is performed 4-6 hours after the optimal fertilization "window", and all IVF patients' oocytes need to ...

AI Technical Summary

Problems solved by technology

[0005] (1) Fertilization is a complex process of the coordinated development of sperm-egg interaction. Sperm must pass through the cumulus cells, bind to the zona pellucida, and then pass through the zona pellucida into the egg cytoplasm, followed by subsequent events such as depolymerization of sperm nuclei. Any obstacle in the process may cause fertilization failure

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