Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food

Abstract

The invention provides a method for quickly detecting nitrates in food, the detection steps are as follows: 1) prepare sodium nitrate aqueous solution whose concentration changes successively, as a standard solution, add citric acid, anhydrous sodium sulfate, naphthaleneethylenediamine hydrochloride mixed 2) Add pure water to the sample, shake and extract to obtain the sample treatment solution; 3) Take the above sample treatment solution , add the powder reagent mixed by citric acid, anhydrous sodium sulfate, naphthalene ethylenediamine hydrochloride etc. in a certain proportion, leave standstill after mixing, obtain the sample color developing solution with color; 4) compare the color of the sample color developing solution with The colors of each standard chromogenic solution are compared to obtain the nitrate content in the tested sample. The invention can quickly and easily detect the content of nitrate in the food, has high sensitivity, and is suitable for rapid on-site determination of non-professionals.

Description

technical field [0001] The invention relates to the technical field of food analysis and testing, in particular to a color card and kit for rapid detection of nitrate in food based on chemical reaction. Background technique [0002] In modern food safety testing, the significance of nitrate detection is very important. Nitrate itself is not harmful, but it will be reduced to nitrite under the action of microorganisms in the human body. Under certain conditions, nitrite and protein can form strong carcinogens - nitroso compounds. Seriously endanger human health. Studies have shown that 80% of the nitrate in the human body comes from vegetables, and in my country, a large amount, excessive or unreasonable use of chemical fertilizers, especially nitrogen fertilizers, on crops every year has caused serious nitrate pollution in fruits and vegetables, especially leafy vegetables , which has caused serious harm to my country's food industry. Research and development of new rapid,...

AI Technical Summary

Problems solved by technology

This method must be operated in a laboratory, and the operation is cumbersome and time-consuming; cadmium can volatilize and has great toxicity to the human body

The reagents used in the enzymatic method are not easy to store and are not suitable for on-site detection operations outside the laboratory

The instruments used in chromatography are expensive, and the consumption of reagents is large, and professional testing personnel are required to operate, so they cannot be popularized

[0004] A rapid nitrate detection agent (patent number: 201310577915.4) is suitable for detecting various media with a nitrate content between 0.01 and 0.5 mg / L, and the minimum limit in the food pollutant limit standard (GB 2762-2012) is 45mg / L in mineral water, and up to 100mg / kg in infant food, this rapid nitrate detection kit cannot detect samples with high nitrate content