A method for detecting polymethoxylated flavonoids
A polymethoxyflavonoid and spectral detection technology is applied in the field of detecting polymethoxyflavonoids, and can solve the problems of inability to detect mixtures with complex components and the like
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Embodiment 1
[0048] Embodiment 1, the detection of polymethoxylated flavonoids in the tangerine peel of Qingjian
[0049] according to figure 1 The process shown in the test is carried out.
[0050] (1) Peel off the satsuma mandarin pulp, cut the peel into thin strips, add liquid nitrogen, keep the peel in liquid nitrogen for 3 minutes, and grind the frozen peel into powder with a powder machine.
[0051] (2) Weigh 100mg of citrus peel dry powder in a clean beaker, then add 25mL of ultrapure water, shake and mix, then transfer to a clean liquid-liquid extraction bottle, then add 2 times the volume of ethyl acetate (50mL), Seal the bottle, vibrate and mix well, and let it stand for 2 minutes. The ethyl acetate phase and the water phase are separated due to different densities. The upper layer is the ethyl acetate phase, and the lower layer is the water phase; then the lower water phase is discharged to remove the citrus peel. Water-soluble pigments, polysaccharides, proteins, glycosides a...
Embodiment 2
[0055] Example 2. Sample detection of metabolites in mice fed nobiletin
[0056] according to figure 1 The process shown in the test is carried out.
[0057] (1) AJ mice were fed in metabolic cages, and AIN-76A mice were given standard food (adding 0.1% 5-desmethylnobiletin). The food jars were regularly replenished every day and all the feces samples of the mice were collected within a week, and the feces samples were pulverized with a powder machine.
[0058] (2) Weigh 100mg of mouse feces sample into a clean beaker, then add 25mL of ultrapure water, shake and mix well, transfer to a clean liquid-liquid extraction bottle, and then add 2 times the volume of ethyl acetate (50mL) , seal the bottle, vibrate and mix well, and then let it stand for 2 minutes. The ethyl acetate phase and the water phase are layered due to different densities. The upper layer is the ethyl acetate phase, and the lower layer is the water phase; then the lower water phase is discharged to remove fece...
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