A method for detecting polymethoxylated flavonoids
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A polymethoxyflavonoid and spectral detection technology is applied in the field of detecting polymethoxyflavonoids, and can solve the problems of inability to detect mixtures with complex components and the like
Active Publication Date: 2019-08-20
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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However, SERS is not an effective separation technology. It is usually impossible to detect complex mixtures when used alone. Therefore, it is necessary to realize the separation and detection of complex samples by combining with other separation technologies.
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Embodiment 1
[0048] Embodiment 1, the detection of polymethoxylated flavonoids in the tangerine peel of Qingjian
[0049] according to figure 1 The process shown in the test is carried out.
[0050] (1) Peel off the satsuma mandarin pulp, cut the peel into thin strips, add liquid nitrogen, keep the peel in liquid nitrogen for 3 minutes, and grind the frozen peel into powder with a powder machine.
[0051] (2) Weigh 100mg of citrus peel dry powder in a clean beaker, then add 25mL of ultrapure water, shake and mix, then transfer to a clean liquid-liquid extraction bottle, then add 2 times the volume of ethyl acetate (50mL), Seal the bottle, vibrate and mix well, and let it stand for 2 minutes. The ethyl acetate phase and the water phase are separated due to different densities. The upper layer is the ethyl acetate phase, and the lower layer is the water phase; then the lower water phase is discharged to remove the citrus peel. Water-soluble pigments, polysaccharides, proteins, glycosides a...
Embodiment 2
[0055] Example 2. Sample detection of metabolites in mice fed nobiletin
[0056] according to figure 1 The process shown in the test is carried out.
[0057] (1) AJ mice were fed in metabolic cages, and AIN-76A mice were given standard food (adding 0.1% 5-desmethylnobiletin). The food jars were regularly replenished every day and all the feces samples of the mice were collected within a week, and the feces samples were pulverized with a powder machine.
[0058] (2) Weigh 100mg of mouse feces sample into a clean beaker, then add 25mL of ultrapure water, shake and mix well, transfer to a clean liquid-liquid extraction bottle, and then add 2 times the volume of ethyl acetate (50mL) , seal the bottle, vibrate and mix well, and then let it stand for 2 minutes. The ethyl acetate phase and the water phase are layered due to different densities. The upper layer is the ethyl acetate phase, and the lower layer is the water phase; then the lower water phase is discharged to remove fece...
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Abstract
The invention discloses a method for detecting polymethoxylated flavonoi ingredients. The method comprises the following steps that an organic solvent and water are adopted for extracting a sample to be detected, and an organic solvent phase is collected; the concentrated organic solvent phase is subjected to two-dimensional thin layer chromatography separation, and dispersion points corresponding to the polymethoxylated flavonoi ingredients are labeled; sol is sprayed to the dispersion points adopted for two-dimensional thin layer chromatography on a normal phase thin layer chromatography plate for surface enhanced Raman scattering spectrum detection, and the spectral intensity of the ingredients corresponding to the dispersion points is obtained; the concentration of the polymethoxylated flavonoi ingredients in the sample is obtained according to the spectral intensity and the linear relation between the concentrations of all ingredients and the Raman scattering spectral intensity. The PMFs ingredients and content in the complex sample can be detected conveniently and fast through a liquid-liquid extraction-thin layer chromatography-surface enhanced Raman scattering combining method, and the method has potential application value on fast detection of PMFs in citrus food and other various samples.
Description
technical field [0001] The invention relates to the technical field of rapid detection of biologically active substances in complex components of food sources, in particular to a method for detecting polymethoxylated flavonoids. Background technique [0002] Citrus is the largest fruit in the world, and its planting area and output both rank first among all fruits. Citrus peel is an important source of traditional Chinese medicines such as tangerine peel, green peel, and aurantium citrus, among which flavonoids are one of the main functional components. Polymethoxyflavones (Polymethoxyflavones, PMFs) exist almost exclusively in citrus, with the highest content in the peel, and the content of various PMFs in orange peel is 0.01% to 0.05%. PMFs use 2-phenylchromone as the core, C 6 -C 3 -C 6 For the basic skeleton structure, in C 4 Has a carbonyl group and has 2 or more -OCH 3 . Almost all PMFs have antioxidant activity and the ability to eliminate free radicals, making...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/40G01N21/65
Inventor 郑金铠张晔赵成英肖航田桂芳赵少杰杨莹张慧娟
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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