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Fermentation medium for improving erythromycin yield and method using the same

A fermentation medium and fermentation culture technology, applied in the field of microorganisms, can solve the problems that limit the development of erythromycin fermentation industry, and achieve the effects of simple scheme, good genetic stability and increased yield

Active Publication Date: 2017-01-18
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, after years of strain selection and process optimization, the fermentation unit gradually increased. At present, the domestic average fermentation unit can reach 8000μg / mL, and the lower fermentation unit is still an important factor restricting the development of my country's erythromycin fermentation industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The invention provides a method for preparing erythromycin by fermenting Saccharopolyspora rubrum BJX001 (preservation number CGMCC No.4767), comprising the following steps:

[0030] Step (1): inoculate Saccharopolyspora red BJX001 in the slant culture medium, the components of the culture medium are: corn starch 12g / L, corn steep liquor 6g / L, magnesium sulfate heptahydrate 1g / L , calcium carbonate 3g / L, agar powder 22g / L, the balance is water, adjust pH to 7.2; Cultivate until spores mature, prepare seed liquid, and set aside;

[0031] Step (2): Inoculate the seed liquid in a fermentation medium to carry out fermentation culture, and the components of the fermentation medium are: cornstarch 50g / L, soybean cake powder 20g / L, corn steep liquor 10g / L, yeast powder 5g / L, potassium dihydrogen phosphate 0.5g / L, sodium chloride 5g / L, soybean oil 5g / L, calcium carbonate 5g / L, amylase 0.03g / L, the balance is water, pH 7.0; fermentation culture is Vibration culture, and the rot...

Embodiment 2

[0035] This example specifically provides a method for preparing erythromycin by fermenting Saccharopolyspora rubrum BJX001. Contains the following steps:

[0036] Step (1): inoculate Saccharopolyspora red BJX001 in the slant culture medium, the components of the culture medium are: corn starch 12g / L, corn steep liquor 6g / L, magnesium sulfate heptahydrate 1g / L , calcium carbonate 3g / L, agar powder 24g / L, the balance is water, adjust pH to 7.5; Cultivate until the spores mature, prepare seed liquid, standby;

[0037] Step (2): Inoculate the seed liquid of the mold strain into a fermentation medium to carry out fermentation culture, and the components of the fermentation medium are: cornstarch 40g / L, soybean cake powder 24g / L, corn steep liquor 8g / L , yeast powder 4g / L, potassium dihydrogen phosphate 0.4g / L, sodium chloride 6g / L, soybean oil 4g / L, calcium carbonate 5g / L, amylase 0.01g / L, the balance is water, and the pH is 6.5; The fermentation culture is a vibration culture, ...

Embodiment 3

[0041] This example specifically provides a method for preparing erythromycin by fermenting Saccharopolyspora rubrum BJX001. Contains the following steps:

[0042] Step (1): inoculate Saccharopolyspora rubrum BJX001 in the slant culture medium, the components of the culture medium are: cornstarch 14g / L, corn steep liquor 7g / L, magnesium sulfate heptahydrate 1.2g / L L, calcium carbonate 3.5g / L, agar powder 24g / L, the balance is water, adjust pH to 7.0; Cultivate until spores mature, prepare seed liquid, and set aside;

[0043] Step (2): Inoculate the seed liquid of the mold strain into a fermentation medium to carry out fermentation culture, and the components of the fermentation medium are: cornstarch 60g / L, soybean cake powder 24g / L, corn steep liquor 12g / L , yeast powder 6g / L, potassium dihydrogen phosphate 0.6g / L, sodium chloride 6g / L, soybean oil 6g / L, calcium carbonate 6g / L, amylase 0.02g / L, the balance is water, and the pH is 7.5; The fermentation culture is a vibration...

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Abstract

The invention relates to a fermentation medium for improving an erythromycin yield and a method using the same, belongs to the field of microbes and provides a Saccharopolyspora erythraea fermentation medium and a method for preparing erythromycin through the medium. Through use of a proper amount of amylase in the fermentation medium, optimization of components of a bacterial strain medium and a fermentation medium and control of other culture conditions, an erythromycin yield is improved. The fermentation medium has an erythromycin yield of 11200 micrograms per milliliter, has a simple formula, has a low cost and is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of microorganisms, and specifically provides a fermentation medium for Saccharopolyspora rubrum and a method for preparing erythromycin using the medium. Background technique [0002] Erythromycin is a class of 14-membered macrolide antibiotics with similar antibacterial spectrum to penicillin, especially antibacterial activity against Gram-positive bacteria, acid-fast bacilli, Rickettsia and large viruses , is the drug of choice for the treatment of diseases caused by drug-resistant Staphylococcus aureus infection and hemolytic streptococcal infection. Its mechanism of action is mainly to combine with the 50S subunit of ribonucleosomes, inhibit peptidyltransferase, affect the translocation process of ribonucleosomes, hinder the growth of peptide chains, inhibit the synthesis of bacterial proteins, and thus play an antibacterial role . [0003] At present, there are nearly 40 domestic enterprises with approval docum...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/01
Inventor 周贤龙石怀月刘静
Owner 牡丹江佰佳信生物科技有限公司
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