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Graphene and protein composite film based on non-covalent modification and preparation method thereof

A non-covalent modification and composite film technology, which is applied in chemical instruments and methods, lamination, coating, etc., can solve the problems that it is difficult to meet the actual needs of graphene, the adsorption amount is small, and the adsorption amount of coupled molecules cannot be controlled. , to achieve good application prospects, uniform film, easy to achieve the effect

Active Publication Date: 2017-01-18
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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Problems solved by technology

The coupling molecules of 1-pyrenebutanoic acid succinimidyl ester and NHSester tripod are sensitive to light and humidity, and the adsorption amount of peptide chains and BSA on the surface of graphene is small, and the adsorption amount of coupling molecules is usually uncontrollable, which is difficult to meet the needs of graphene applications. Practical needs in biosensors

Method used

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  • Graphene and protein composite film based on non-covalent modification and preparation method thereof
  • Graphene and protein composite film based on non-covalent modification and preparation method thereof

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Embodiment 1

[0025] Such as figure 1 Shown is a schematic diagram of the structure of protein films with different nanoscale thicknesses formed on the surface of single-layer graphene on a silica substrate. Transfer the single-layer graphene film grown by chemical vapor deposition to the surface of the silicon dioxide substrate, remove the photoresist on the surface of the graphene film and leave the single-layer graphene on the surface of the silicon dioxide substrate; the protein solution Add it dropwise to the heat inactivation treatment of single-layer graphene to form a protein film on the surface of single-layer graphene, and obtain a non-covalently modified graphene-protein composite film.

[0026] Dissolve the BSA solution with a concentration of 1.8mM in deionized water, set the temperature of heating inactivation at 75°C, and control the time of protein inactivation at 2min, 4min and 6min, respectively, which can form different nanoscale thickness on the surface of graphene. Pr...

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Abstract

The invention relates to a graphene and protein composite film based on non-covalent modification and a preparation method thereof. The composite film comprises a silica substrate, single-layer graphene and a protein film. The preparation method comprises the following steps: transferring the single-layer graphene film grown through chemical vapor deposition onto the surface of the silica substrate, removing a photoresist on the surface of the graphene film to leave single-layer graphene on the surface of the silica substrate; and dropwise adding the a protein solution onto the surface of the single-layer graphene and carrying out heat inactivation treatment at 60-90 DEG C for 1-10 min to form a protein film on the surface of the single-layer graphene. Non-covalent modification of the protein film is realized on the surface of graphene through the heat inactivation method. Thickness of the graphene-protein film can be controlled to nanoscale. The film is uniform. The preparation method is simple to operate and easy to realize. The protein film surface has amino group and carboxyl group and can be used as a platform for follow-up biomolecule diagnosis. The product has a good application prospect.

Description

technical field [0001] The invention belongs to the field of graphene biosensors, in particular to a graphene protein composite film based on non-covalent modification and a preparation method. Background technique [0002] Graphene is a two-dimensional material with a single-layer carbon atomic structure. Due to its stable chemical structure and excellent optical, mechanical and electrical properties, graphene is an ideal material in the field of nano-bioelectric detection. Graphene devices are used to detect proteins, DNA, nerve cell action potentials, and bacteria. In the construction process of graphene biosensors, the biomolecular modification of the graphene surface is a crucial link. The modification methods of graphene surface can generally be divided into covalent modification and non-covalent modification. Covalent modification of graphene is usually stable but will damage the electrical properties of graphene, while non-covalent modification can usually effectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B32B9/00B32B9/04B32B33/00B32B37/00C08J5/18G01N23/227C08L89/00
CPCB32B9/007B32B9/04B32B33/00B32B37/00B32B2255/26C08J5/18C08J2389/00G01N23/2273
Inventor 毛红菊周麟马云飞金庆辉赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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