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Method for determining and screening differential metabolites of laryngeal cancer serum based on inverted phase chromatography time-of-flying mass spectrum

A technology of time-of-flight mass spectrometry and reversed-phase chromatography, applied in the field of physical and chemical testing of serum, can solve the problems of cumbersome collection of urine samples, inability to fully represent subjects, and error-prone, and achieve simple pre-treatment, high sensitivity, and rapid repeatability sexual effect

Active Publication Date: 2017-01-11
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT +1
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Problems solved by technology

However, for clinical diagnosis, blood is often used, because the collection of 24-hour urine samples is cumbersome and error-prone, and the concentration of compounds in morning urine is easily affected by the liquids (such as beverages, water, etc.) that the subjects ingest. impact, cannot fully represent the state of the subject at that time, and the blood flowing through the subject's body can well reflect the subject's physical condition
At present, serum has been widely used in the study of differential metabolites in lung cancer and nasopharyngeal carcinoma, but the study of differential metabolites in laryngeal cancer has not been reported

Method used

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  • Method for determining and screening differential metabolites of laryngeal cancer serum based on inverted phase chromatography time-of-flying mass spectrum
  • Method for determining and screening differential metabolites of laryngeal cancer serum based on inverted phase chromatography time-of-flying mass spectrum

Examples

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example 1

[0030] 1. Instruments and reagents: The liquid chromatograph used in the experiment is Dionex’s UltiMate 3000, including liquid phase pump (HPG-3400 SD), autosampler (WPS-3000 SL) and column thermostat (TCC-3000 SD), All samples were used ACQUITY UPLC produced by Water Company ® BEH column (2.1 × 150 mm, 1.7 µm) was used for separation, and the column temperature was 50 ºC.

[0031] Chromatographic grade formic acid, with a purity of 98%, was purchased from TEDIA; acetonitrile, with a purity of 99.9%, was purchased from DUKSAN PURE CHEMIVALS, Korea; 1-hexadecanoyl-sn-glycerol-phosphocholine , with a purity of more than 99%, purchased from Sigma-Aldrich; the experimental water was deionized water.

[0032] 2. Sample processing: thaw serum at room temperature, pipette 400 µL of serum, add 1200 µL of methanol, vortex mix for 15 s, then centrifuge in a centrifuge at 15800 g for 15 min, pipette 370 µL of supernatant in 2 mL centrifuge tube, then dried by centrifugation in a va...

example 2

[0037] Example 2: According to the method described in Example 1, 22 laryngeal cancer volunteers and 26 healthy volunteers were selected for serum samples, and a differential metabolite was identified as 1-hexadecanoyl-sn-glycerol-phosphate choline.

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Abstract

The invention relates to a method for determining and screening differential metabolites of laryngeal cancer serum based on an inverted phase chromatography time-of-flying mass spectrum. The method is characterized by comprising the steps of settling protein from serum by virtue of an organic solvent, namely methanol, carrying out centrifugation and vacuum centrifugal drying, redissolving the serum into 200 microliters of pure water, introducing the solution into an inverted phase chromatography time-of-flying mass spectrometer for determination, and carrying out peak alignment, correction and standardization, multivariate statistics analysis and spectrum library searching, so as to find differential metabolites in serums of laryngeal cancer volunteers and healthy volunteers. The method is a brand-new method for identifying the differential metabolites in the serum, and the variation conditions of the serum metabolites of laryngeal cancer patients can be reflected by the differential metabolites, so that the method will contribute to the diagnosis, clinic treatment and prognosis of the laryngeal cancer and the research of pathogenesis of the laryngeal cancer.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of physical and chemical testing of serum, in particular to a method for measuring and screening differential metabolites in serum of laryngeal cancer based on reversed-phase chromatography time-of-flight mass spectrometry. [0003] Background technique: [0004] The common type of laryngeal cancer is squamous cell carcinoma, which has the characteristics of high incidence and high recurrence rate, and is prone to cervical lymph node metastasis. In the treatment of laryngeal cancer, laryngectomy is the main method of surgery, supplemented by radiotherapy and chemotherapy, but it is not sensitive to chemotherapy, and it is easy to cause problems such as poor pronunciation and swallowing function of patients, and reduced quality of life. The treatment effect of early laryngeal cancer The curative effect of advanced laryngeal cancer is better, but there is still a lack of effective biomarkers for the e...

Claims

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Application Information

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IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 胡清源侯宏卫张小涛陈欢刘勇王安刘彤韩书磊付亚宁
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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