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Serum iodine quantitative determination kit

A quantitative determination and kit technology, applied in the measurement of color/spectral properties, etc., can solve the problems of high consumption of dangerous reagents, expensive experimental instruments, complicated reagent preparation, etc., and achieve high accuracy of test results, long storage time, large The effect of social benefit

Active Publication Date: 2017-01-04
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to digest the serum through the kit, and then utilize the catalytic effect of iodine on the redox reaction of arsenic and cerium to determine the Ce in the reaction system 4+ The change of the absorbance value, using the logarithm of the iodine concentration and the absorbance value to calculate the iodine content in a linear relationship, overcomes the complex reagent preparation, easy pollution, easy decomposition, large amount of dangerous reagents, expensive experimental equipment, and Disadvantages of higher serum volume required

Method used

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  • Serum iodine quantitative determination kit
  • Serum iodine quantitative determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 A kind of kit preparation of serum iodine quantitative determination

[0039] 1. Preparation of 100 μg / mL iodine standard stock solution

[0040] Accurately weigh 0.1686g of potassium iodate that has been dried at 105-110°C to constant weight in a beaker, dissolve it in pure water and transfer it quantitatively into a 1000mL volumetric flask, dilute to the mark with pure water, and store in the dark.

[0041] The standard series of solutions prepared by using the iodine standard stock solution has a good linear relationship and a range of absorbance values ​​during detection, which can meet the detection of iodine content in human and animal serum.

[0042] 2. 70%~72% perchloric acid (digestion solution R 1 ) and 2.0mol / L sodium chlorate solution (digestion solution R 2 ) preparation

[0043] 2.1 Digestion solution selection

[0044] Compared with other samples, serum has a complex base composition, a wide range of inorganic ions, and high protein conte...

Embodiment 2

[0058] Embodiment 2 experimental instrument and equipment

[0059] 1. Constant temperature digestion instrument (temperature control point accuracy 130°C±2°C, temperature difference between wells ≤1°C)

[0060] 2. Spectrophotometer

[0061] 3. Glass test tube: 15mm×100mm or 15mm×120mm

[0062] 4. Stopwatch

Embodiment 3

[0063] Embodiment 3 kit performance index investigation

[0064] According to "GB / T 210.5-2008 Occupational Hygienic Standard Formulation Guidelines Part 5: Determination of Chemical Substances in Biological Materials", the method characteristics of the kit for the quantitative determination of iodine in serum of the present invention are measured, and the results are as follows:

[0065] 1. Prepare the kit as described in Example 1.

[0066] 2. Sample collection and storage

[0067] Use a disposable vacuum blood collection tube to collect no less than 2mL of blood, let it stand at room temperature for 0.5h, centrifuge at 3000r / min for 10min, separate the serum and put it in a polyethylene plastic tube with a stopper, and seal it tightly to prevent moisture from evaporating. It can be stored at room temperature (20°C) for 7 days, at 4°C for 2 months, and sealed and frozen (-20°C) for at least 3 months. Blood samples or serum samples should avoid contact with iodized items du...

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Abstract

The invention discloses a serum iodine quantitative determination kit. Serum samples are digested through an acid digestion method, and the iodine content is calculated by means of catalysis of iodine to an arsenic-cerium oxidation-reduction reaction, determination on changes of the Ce<4+> absorbance value in a reaction system and the condition that the iodine concentration and the logarithm of the absorbance value have a linear relation. Building of the kit mainly comprises work of three aspects of digestion condition selection, reaction system determination and method validation, and the detects that an existing method is complicated in reagent preparation, pollutes the environment and harms health of a detected person, and instruments are expensive are overcome. The method is mainly used for detecting the content of iodine in serum of human bodies and animals, detection can be carried out in provincial level, prefecture level and county and district level disease prevention and control departments in China and medical institutions at various levels conveniently, and requirements of individual iodine nutrition evaluation for disease control and in clinical practice can be met.

Description

technical field [0001] The invention relates to a kit for quantitative determination, in particular to a kit for quantitative determination of serum iodine, which belongs to quantitative detection products in the field of application technology. Background technique [0002] At present, there are more and more people seeking to detect individual iodine nutrition levels. The clinical method is to evaluate a random urine sample to detect iodine levels. However, urinary iodine can only reflect the excretion of iodine in the body, and cannot represent the iodine used by the thyroid gland. The level of biologically active iodine ions and the serum iodine concentration can truly reflect the iodine nutritional status of the body. [0003] At present, there are many quantitative determination methods of serum iodine described in the literature, such as mild acid digestion method to measure total serum iodine content (Hu Mei, Ze Renyongxi, etc., Chinese Journal of Endemic Diseases. 2...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 申红梅陆征张亚平纪晓红姜鹏刘颖刘丽香
Owner HARBIN MEDICAL UNIVERSITY
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