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SNP molecular marker combination universal for salmon and bulltrout and application of combination

A molecular marker and salmon trout technology, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as inbreeding depression, achieve high typing accuracy, avoid linkage disequilibrium, large number of effects

Active Publication Date: 2017-01-04
CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of such genetic tools in the salmon and trout farming industry in my country is still blank, and the parents can only be selected according to the appearance characteristics in the process of seed breeding, and the risk of inbreeding depression is high

Method used

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  • SNP molecular marker combination universal for salmon and bulltrout and application of combination
  • SNP molecular marker combination universal for salmon and bulltrout and application of combination
  • SNP molecular marker combination universal for salmon and bulltrout and application of combination

Examples

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Embodiment 1

[0038] Example 1 Application of SNP molecular markers for salmon and trout for polymorphism assessment of germplasm genetic resources

[0039] As shown in Table 1, the present invention provides a combination of SNP molecular markers for polymorphism assessment of salmon and trout germplasm genetic resources. The combination of SNP molecular markers has a base sequence such as SEQ ID NO: 1-96. The SNP molecular marker combination provided by the invention is used for salmon and trout fish, and the result is accurate, and the SNP molecular marker polymorphism and genetic relationship can be detected with high efficiency, high throughput and simple application.

[0040] A method for evaluating the genetic polymorphism of salmon and trout by using the general SNP molecular marker combination of salmon and trout, comprising the steps of:

[0041] Step 1. Obtain the genomic DNA of the germplasm salmon and trout to be evaluated. Preferably, in the method for polymorphism assessment...

Embodiment 2

[0051] Example 2 Application of common SNP molecular markers for salmon and trout in the evaluation of actual population seed production

[0052] In order to verify the feasibility of inferring the whole set of SNP molecular markers in actual populations and its accuracy in seed production evaluation, the inventors selected 48 rainbow trout from 6 populations with different origins for intraspecific phylogenetic identification . See Table 3 for specific sample information.

[0053] A kind of method that utilizes described salmon trout common SNP molecular marker combination to carry out salmon trout fish breeding evaluation, taking rainbow trout as an example, comprises the following steps:

[0054] Step 1. Obtain the genomic DNA of the rainbow trout germplasm to be evaluated. Preferably, in the method for identification of salmon trout seed production, in the first step, the genome is obtained from the blood of the rainbow trout.

[0055] Step 2, obtaining the genotypes of...

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Abstract

The invention discloses an SNP molecular marker combination universal for salmon and bulltrout. The combination comprises 96 SNP molecular markers, and the base sequences of the SNP molecular markers are nucleotide sequences as shown in SEQ ID NO:1 to SEQ ID NO:96. The invention further discloses a method for evaluating genetic polymorphism of the salmon and bulltrout through the SNP molecular marker combination. The method includes the steps of firstly, obtaining genome DNA of the salmon and bulltrout needing to be specially evaluated; secondly, obtaining genotypes of a plurality of SNP molecular marker loci of each salmon or bulltrout; thirdly, analyzing the result of the genotypes of the SNP molecular marker loci of each salmon or bulltrout according to a Mendelian inheritance rule through bioinformatics software, conducting analyzing to obtain typing information of the salmon and bulltrout, judging the genetic relationship between the salmon and bulltrout, and determining whether the salmon and bulltrout are suitable as parents for propagation or not, wherein the SNP molecular markers are contained in the 96 SNP molecular markers. The invention further discloses a method for specially evaluating the salmon and bulltrout through the SNP molecular marker combination.

Description

technical field [0001] The present invention relates to a common SNP molecular marker combination for salmon and trout and its application, in particular to 96 marker combinations and primer sequences, in particular to an application for polymorphism assessment of salmon and trout germplasm genetic resources, and especially It relates to an application of salmon and trout seed production evaluation. Background technique [0002] Salmon and trout are collectively referred to as salmon and trout, belonging to Osteichthyes, Actinopterygii, Salmoniformes, and Salmonidae. There are 7 genera and 36 species in my country. Among them, 4 genera and 8 species are cultivated artificially, and they are important cold-water famous and high-quality aquatic species. Salmon and trout have high requirements for living water quality and can only grow in cool, flowing, and non-polluted waters. Their meat is delicious, high in protein, rich in unsaturated fatty acids, and without intermuscular ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张瀚元赵紫霞许建徐鹏白庆利
Owner CHINESE ACAD OF FISHERY SCI
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