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Stevia phenol extract and application thereof in preparation of bacteriostatic product

The technology of steviol phenol and extract is applied to steviol phenol extract and its application field in preparing antibacterial products, which can solve problems such as environmental pollution and resource waste, and achieve rich source of raw materials and good antibacterial effect. , good stability effect

Active Publication Date: 2017-01-04
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stevia phenols exist as impurities in the production process of steviol glycosides, and are separated from steviosides in the flocculation process, and enter the flocculation residue as waste, which not only causes waste of resources, but also causes great pollution to the environment

Method used

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  • Stevia phenol extract and application thereof in preparation of bacteriostatic product
  • Stevia phenol extract and application thereof in preparation of bacteriostatic product
  • Stevia phenol extract and application thereof in preparation of bacteriostatic product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example provides an experiment on the antibacterial effect of stevia phenolic extracts.

[0028] 1.1 Materials and reagents

[0029] Stevia phenolic extract; MH(A) medium, MH(B) medium, Beijing Aoboxing Biotechnology Co., Ltd.; Staphylococcus aureus (Staphylococcus aureus) AS1.89, Bacillus subtilis (Bacillus subtilis) AS1 .1849, Bacillus cereus (Bacillus cereus) AS1.1846, Institute of Microbiology, Chinese Academy of Sciences; other reagents are domestic analytical grade.

[0030] 1.2 Instruments and equipment

[0031] ZWP-A1230 constant temperature and humidity incubator, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.; SW-CJ-2FD double-person single-sided purification workbench, Suzhou Purification Equipment Co., Ltd.

[0032] 1.3.1 Activation of strains and preparation of bacterial suspension

[0033] The strains were transferred from the slant to the slant of the fresh medium with an inoculation loop, and each bacterium was cultured in a 37°C...

Embodiment 2

[0049] This example provides a measurement experiment of the time-antibacterial curve of stevia phenolic extracts.

[0050] 2.1 Materials and reagents

[0051] Stevia phenolic extract; MH(B) medium, Beijing Aoboxing Biotechnology Co., Ltd.; Staphylococcus aureus (Staphylococcus aureus) AS1.89, Bacillus subtilis (Bacillus subtilis) AS1.1849, Bacillus cereus (Bacillus cereus) AS1.1846, Institute of Microbiology, Chinese Academy of Sciences; other reagents were of domestic analytical grade.

[0052] 2.2 Instruments and equipment

[0053] SPECTRA MAX 190 continuous wavelength microplate reader, American MD company; HQ45 constant temperature shaker, Wuhan Scientific Instrument Factory, Chinese Academy of Sciences; ZWP-A1230 constant temperature and humidity incubator, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.; SW-CJ-2FD double single Surface purification workbench, Suzhou Purification Equipment Co., Ltd.

[0054] 2.3 Experimental process

[0055] 2.3.1 Pre...

Embodiment 3

[0065] Study on Antibacterial Stability of Stevia Phenolic Extracts

[0066] 3.1 Materials and reagents

[0067] Stevia phenolic extract; MH(A) medium, MH(B) medium, Beijing Aoboxing Biotechnology Co., Ltd.; Staphylococcus aureus (Staphylococcus aureus) AS1.89, Bacillus subtilis (Bacillus subtilis) AS1 .1849, Bacillus cereus (Bacillus cereus) AS1.1846, Institute of Microbiology, Chinese Academy of Sciences; other reagents are domestic analytical grade.

[0068] 3.2 Instruments and equipment

[0069] HQ45 constant temperature shaking table, Wuhan Scientific Instrument Factory, Chinese Academy of Sciences; ZWP-A1230 constant temperature and humidity incubator, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.; SW-CJ-2FD double single-sided purification workbench, Suzhou Purification Equipment Co., Ltd.; HH- 4 Digital constant temperature water bath, Jintan Ronghua Instrument Manufacturing Co., Ltd., Jiangsu Province; pH400 pH meter, Anlaisi Instrument Technology ...

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Abstract

The invention discloses a stevia phenol extract and application thereof in preparation of a bacteriostatic product, and belongs to the technical field of plant extracts. The stevia phenol extract is a polyphenol compound taking flavone and chlorogenic acid as main ingredients. The stevia phenol extract has a bacteriostatic effect on three kinds of G<+> bacteria including staphylococcus aureus, bacillus cereus and bacillus subtilis, and the bacteriostatic activity is enhanced along with increasement of the concentration of the stevia phenol extract. The stevia phenol extract still has the good bacteriostatic activity after being treated at high temperature, the bacteriostatic activity on the bacillus cereus is reduced by increasing the pH value and conducting ultraviolet treatment, and the bacteriostatic activity of the stevia phenol extract on the staphylococcus aureus and the bacillus subtilis can be enhanced through Na<+>, K<+> and Ca<2+>. The stevia phenol extract has the good bacteriostatic activity and bacteriostatic stability and has wide development and utilization prospects by being used as the bacteriostatic product.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and relates to a stevia phenolic extract and its application in the preparation of antibacterial products. Background technique [0002] Stevia rebaudiana (Stevia rebaudiana) is a perennial herb of Compositae, native to Paraguay and Brazil in South America, and is mainly used as a raw material for the production of stevia. The sweetness of stevia is 150-300 times that of sucrose, but the calories are only 1 / 300 of sucrose. In recent years, steviol glycosides have been widely used in food, beverage and daily chemical products industries, and stevia has become the third largest sugar source after sugarcane and sugar beet. In addition to steviol glycosides, Stevia rebaudiana also contains flavonoids, chlorogenic acid and other ingredients, and these ingredients have important biological activities, such as antibacterial, lowering blood pressure, lowering blood lipids, etc. It has been used a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P31/04A23L3/3472
CPCA23L3/3472A61K36/28A61K2236/331A61K2236/39A61K2236/51A61K2236/55
Inventor 徐美利赵磊杨香瑜安晓东李乾丽连运河王成涛
Owner CHENGUANG BIOTECH GRP CO LTD
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