Application of lactobacillus plantarum in preparing antiallergic product
A technology of Lactobacillus plantarum, food allergy, applied in the direction of Lactobacillus plantarum, bacteria used in food preparation, application, etc., to achieve the effect of preventing immune system diseases, good industrial prospects, and low price
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Embodiment 1
[0100] Embodiment 1, the acquisition of plantarum lactobacillus SC9
[0101] Strain SC9 was isolated from traditional fermented sauerkraut in Northeast China. Physiologically and biochemically identified as Gram-positive bacteria, identified as Lactobacillus plantarum (Lactobacillus plantarum) by API50CH (Mérieux, France) and 16srDNA sequence amplification comparison, named Lactobacillus plantarum (Lactobacillus plantarum) SC9, referred to as Lactobacillus plantarum SC9 .
[0102] Lactobacillus plantarum SC9 was deposited in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University) on October 10, 2008, and the preservation number is CCTCC NO.M 208150.
[0103] Lactobacillus plantarum SC9 has applied for a patent on December 11, 2008 and obtained authorization. The patent number is ZL 2008 10239475.0. The authorization announcement date is January 4, 2012. The authorized announcement number is CN 101748083 B.
Embodiment 2
[0104] Embodiment 2, the antiallergic effect of plantarum lactobacillus SC9
[0105] 1. Preparation of bacterial suspension and allergen
[0106] Suspend Lactobacillus plantarum SC9 with PBS buffer to obtain a bacterial concentration of 10 9 CFU / mL bacterial suspension.
[0107] Mix 1 mL of Freund's adjuvant and 1 mL of 1 mg / mL β-lg solution to obtain the allergen.
[0108] Two, group processing
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