Method for establishing griffinia liboniana bulblet regeneration system by taking stamen as explant
A technology of small bulbs and explants, which is applied in the field of establishment of blue crystal flower small bulb regeneration system, to achieve the effects of improving induction rate and proliferation efficiency, shortening propagation cycle, and high callus induction rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] (1) Extraction and processing of explants
[0040] During the flowering period of the blue crystal flower in June, young flower buds (buds not opened) with a length of 1.5-2.5 cm were picked, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.
[0041] Transfer the refrigerated flower bud material to a beaker, soak in water with a little Tween and detergent added dropwise for 15 minutes, then cover the mouth of the beaker with gauze, and rinse the gauze with running water for 1 hour. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 8min. Reagents are fully exposed.
[0042] Finally, rinse 3 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.
[0043] (2) Explant inoculation and callus induction
...
Embodiment 2
[0056] (1) Extraction and processing of explants
[0057] In the flowering period of the blue crystal flower in June, young flower buds with a length of 1.5-2.5 cm (buds are not opened) are collected, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.
[0058] The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 15 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.
[0059] Finally, rinse 4 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.
[0060] (2) Explant inoculati...
Embodiment 3
[0073] (1) Extraction and processing of explants
[0074] In the flowering period of the blue crystal flower in June, young flower buds with a length of 1.5-2.5 cm (the buds are not opened) are collected, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.
[0075]The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 20 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.
[0076] Finally, rinse with sterile water 5 times, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.
[0077] (2) Explant inocul...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com