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Surface enhanced Raman scattering technology-based composite material and preparation method thereof

A composite material and mixed liquid technology, applied in the field of medicine, can solve problems such as false positives

Active Publication Date: 2016-12-28
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, Raman signal molecules and antibodies are directly modified on the surface of SERS nanoparticles, which will inevitably cause the exposed surface of metal nanoparticles to react with -SH on the antigen, resulting in false positive results

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  • Surface enhanced Raman scattering technology-based composite material and preparation method thereof
  • Surface enhanced Raman scattering technology-based composite material and preparation method thereof
  • Surface enhanced Raman scattering technology-based composite material and preparation method thereof

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preparation example Construction

[0236] The present invention also provides a kind of preparation method of described composite material, described method comprises the steps:

[0237] 1) provide the first component and the second component, wherein,

[0238] The first component was prepared as follows:

[0239] a-1) providing microspheres and a first target molecule;

[0240] a-2) reacting the microspheres and the first target molecule under the action of a catalyst to obtain the first component;

[0241] The second component was prepared as follows:

[0242] b-1) providing the first mixed solution, the second mixed solution, the third mixed solution and the fourth mixed solution, wherein,

[0243] The first mixed liquid contains a first solvent and the noble metal particles;

[0244] The second mixed solution contains a second solvent and the Raman signal molecule;

[0245] The third mixed liquid contains a third solvent and the polymer;

[0246] The fourth mixed solution contains a fourth solvent and...

Embodiment 1

[0294] Preparation of Composite Material 1

[0295] (1) Preparation of gold nanospheres (AuNPs) with SERS function

[0296] Add 10mL of 5mM chloroauric acid into 30mL of ultrapure water, heat to boiling, then add 10mL of 1% trisodium citrate, the color of the reaction solution turns purple, stop the reaction, cool naturally to room temperature, the solution turns wine red, and then AuNPs were prepared and stored at 4°C.

[0297] (2) Preparation of gold nanospheres (AuNP-MBA) linked with Raman signaling molecules

[0298] Dissolve 4-mercaptobenzoic acid (4-MBA) in tetrahydrofuran solvent to prepare a tetrahydrofuran solution of 4-mercaptobenzoic acid with a concentration of 50 mM.

[0299] Take 3mL of the gold nanospheres described in (1), add them into 9mL of ultrapure water, mix well, then add 30.0μL of 4-mercaptobenzoic acid solution, and after a certain period of reaction, the AuNP-MBA material is prepared and stored at 4°C .

[0300] (3) Reduction of polymer bovine ser...

Embodiment 2

[0322] Preparation of Composite Material 2

[0323] (1) Preparation of gold nanorods (AuNRPs) with SERS function

[0324]The first step is the preparation of gold seeds. Mix 7.5mLCTAB and 0.5mM 5mM chloroauric acid evenly, and then quickly add 0.6mL0.01M sodium borohydride. After the solution turns brown, the gold seeds can be prepared and stored at 4°C.

[0325] The second step, preparation of growth solution, mix 100mL0.1MCTAB and 0.1mM20mM silver nitrate, add 12mL5mM chloroauric acid and stir evenly, then add 0.48mL ascorbic acid aqueous solution, after the solution becomes colorless, add 0.2mL gold species, avoid light reaction , when the color changes to dark blue, gold nanorods (AuNRPs) with SERS function can be prepared.

[0326] (2) Linking Raman signal molecules on the surface of nanoparticles, reduction of disulfide bonds of biomolecules, encapsulation of reduced biopolymers on the surface of nanoparticles, coupling of human prostate-specific antibodies on the surfa...

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Abstract

The invention relates to a surface enhanced Raman scattering technology-based composite material and a preparation method thereof. The composite material comprises microspheres modified with first target molecules on the surfaces and a second component modified with Raman signal molecules and second target molecule-modified polymers on the surface. In a solution containing a substance to be detected, the substance to be detected, the microspheres and the second component are bonded to the second target molecules through the first target molecules so that the composite material which can be easily precipitated through centrifugation is obtained. The invention also discloses the preparation method and a use of the composite material. The composite material can be used for detecting a human prostate-specific antigen (PSA), a human ferrohemoglobin (Hb), a procalcitonin (PCT) or disease markers (such as tumor markers, cardiovascular disease markers, senile dementia markers, mycoplasma and chlamydia), can effectively reduce false positive and false negative effects and has advantages of high sensitivity, simpleness, fastness and low cost.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a composite material based on surface-enhanced Raman scattering technology and a preparation method thereof Background technique [0002] Specific molecular recognition, such as antigen-antibody, biotin-avidin, DNA-DNA or RNA, is important for biological analysis. Among them, the specific interaction of antigen-antibody is the most widely used in the detection of human diseases. The human immune system can produce different kinds of antigens on the surface of abnormal cells, and the detection of antigens can achieve the effect of diagnosing diseases. However, the current methods for clinical antigen detection include serum detection, enzyme-linked immunosorbent assay, etc., all of which have the disadvantages of complicated operation, long time-consuming, and low sensitivity when detecting low-concentration antigens. In addition, early diagnosis of human diseases plays a vital...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 沈折玉吴爱国武小侠张玉杰阮慧敏
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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