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Method for measuring biogenic amine in tobaccos

A determination method and biogenic amine technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve problems such as complex operation, and achieve the effects of high sensitivity, low detection limit, and simple and convenient analysis.

Active Publication Date: 2016-12-21
CHINA TOBACCO HENAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), thin-layer chromatography (TLC) and gas chromatography (GC) are generally used for the detection of biogenic amines. The above-mentioned detection methods all need to derivatize the sample, and the operation is relatively complicated.

Method used

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  • Method for measuring biogenic amine in tobaccos
  • Method for measuring biogenic amine in tobaccos
  • Method for measuring biogenic amine in tobaccos

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Embodiment 1

[0032] A method for determining biogenic amines in tobacco, including the following steps,

[0033] (1) Prepare mixed series of standard working solutions: prepare mixed series of standard working solutions with concentration gradient and internal standards;

[0034] (2) Sample extraction: Add 0.1g of pulverized tobacco into a formic acid aqueous solution (2vol%)-acetonitrile mixture (the volume ratio of formic acid aqueous solution and acetonitrile is 4:1) according to a solid-liquid ratio of 1g:20mL, and add 100μL 500ng / mL internal standard 1,7-heptanediamine solution, centrifuge at 13000 rpm for 4min after mixing, take out the supernatant, and add the filter residue to 2mL formic acid aqueous solution (2vol%)-acetonitrile mixture (volume ratio of formic acid aqueous solution and acetonitrile) 4:1) Mix well, centrifuge at 13000 rpm for 4 minutes, take the supernatant, and combine the supernatants of the two extractions to obtain the sample extract;

[0035] (3) Purification of so...

Embodiment 3

[0060] Take 5 tobacco leaf samples that have been re-cured and aged for 1-2 years, and analyze the samples according to the determination method of the present invention. The sample analysis results are shown in Table 5.

[0061] Table 5 Contents of biogenic amines in tobacco leaf samples

[0062]

[0063]

[0064] Note: "-" means that it is not detected in the sample.

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Abstract

The invention belongs to a method for measuring biogenic amine in tobaccos. The method includes that liquid-liquid extraction-tandem solid-phase extraction are used as pretreatment means, and the seven types of biogenic amine (including putrescine, cadaverine, histamine, tyramine, spermine, spermidine and tryptamine) in the tobaccos are measured by the aid of high-performance liquid chromatography-mass spectrometry / mass spectrometry (HPLC-MS / MS) processes. Compared with the literature, the method has the advantages that the method suitable for measuring the biogenic amine in the tobaccos is created; the created method is simple and reliable and can be applied to detecting the biogenic amine in the tobaccos; samples can be easily, convenient and quickly analyzed by the aid of the method, and the method is low in detection limit, high in sensitivity and good in repeatability and recovery rate.

Description

Technical field [0001] The invention belongs to the technical field of detection of biogenic amines in tobacco, and specifically relates to a method for determining biogenic amines in tobacco. Background technique [0002] Biogenic amines are a general term for a kind of biologically active nitrogen-containing low molecular weight basic organic compounds. They are the precursors of the organism's own synthesis of hormones, nucleotides, and proteins, which can be regarded as 1-3 hydrogens in the ammonia molecule. A low-molecular-weight alkaloid formed after the atom is substituted by an alkyl or aryl group. Biological amines can be divided into three categories according to their structure: aliphatic (putrescine, cadaverine, spermine, spermidine, etc.), aromatic amines (tyramine, β-phenethylamine, etc.) and heterocyclic amines (histamine, Tryptamine, etc.). [0003] Biogenic amines are mainly formed by the decarboxylation of amino acids, and a small part of them are formed by the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 付瑜锋田海英段鹍于建春王宏伟李耀光王宝林郝辉胡少东王鹏飞
Owner CHINA TOBACCO HENAN IND
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