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Detection method for esterifying power of distillers yeast

A detection method and esterification technology, applied in the field of brewing, can solve the problems of parallel samples and detection result deviation, low detection result accuracy, cumbersome determination procedures, etc., to improve work efficiency, facilitate rapid detection, and reduce sample distillation The effect of the process

Active Publication Date: 2016-12-14
SICHUAN JIANNANCHUN GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the mensuration of traditional esterification power is to carry out esterification power detection with single hexanoic acid as esterification substrate, be to adopt mass percent concentration to be 1% hexanoic acid and 20% ethanol solution when preparing hexanoic acid solution, but 1 % hexanoic acid cannot be completely dissolved in 20% ethanol solution, and the parallel sample and test results will cause great deviation when performing esterification test; and the traditional method is to distill the esterification liquid first and then take the distillation The liquid is saponified, and the esters will be lost during the distillation process. Therefore, the traditional method has the disadvantages of low accuracy of detection results, cumbersome determination procedures, and high energy consumption.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:

[0023] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 25°C for 20h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;

[0024] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , put it into a boiling water bath for reflux saponification for 25min, titrate with sulfuric acid until the pH of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;

[0025] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterification power=(C 1 V...

Embodiment 2

[0026] Embodiment 2: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:

[0027] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 35°C for 30h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;

[0028] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , placed in a boiling water bath for reflux saponification for 25 to 35 minutes, titrated with sulfuric acid until the pH value of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;

[0029] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterificati...

Embodiment 3

[0030] Embodiment 3: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:

[0031] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 30°C for 24h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;

[0032] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , put it into a boiling water bath for reflux saponification for 30min, titrate with sulfuric acid until the pH of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;

[0033] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterification power=(C 1 V...

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Abstract

The invention discloses a detection method for an esterifying power of distillers yeast. The detection method comprises sample pretreatment, titration and calculation. According to the invention, a mixed acid of caproic acid, acetic acid and lactic acid is used as an esterifying substrate to detect the esterifying power, and the caproic acid, acetic acid and lactic acid are directly prepared in absolute ethanol to prepare a caproic acid-acetic acid-lactic acid ethanol solution of which the concentration is 5%, so that acids which are insoluble in water are fully dissolved, detection results of the method are good in reproducibility and experimental data of the method are reliable, and the detection results can more truly reflect the esterifying and aroma producing abilities of a starter; according to the method, saponification is directly performed on an esterifying liquid to avoid loss of produced ester matters and make the detection results more accurate, and further save energy and lower consumption, simplify detection procedures and improve work efficiency; the method disclosed by the invention shortens the detection time, and is more conducive to rapid detection principles and suitable for industrialized large-scale detection.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a method for detecting the esterification power of distiller's yeast. Background technique [0002] Liquor is a precious heritage left over from the five thousand years of Chinese civilization and an important component of Chinese traditional culture. Daqu is the driving force of brewing fermentation, and it is a composite carrier rich in bacteria, enzymes and substances needed for brewing. Daqu is used as a saccharification starter and flavoring agent in liquor brewing. Therefore, the quality of Daqu has a great influence on the yield and quality of liquor. [0003] With the advancement of science and technology and the development of the commodity economy, the advantages of regional resources and technological advantages of China's famous liquor have become increasingly prominent, and the phenomenon of exporting and commercializing and industrializing large-scale ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
CPCG01N31/16
Inventor 徐姿静徐占成唐清兰刘孟华
Owner SICHUAN JIANNANCHUN GRP CO LTD
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