Detection method for esterifying power of distillers yeast
A detection method and esterification technology, applied in the field of brewing, can solve the problems of parallel samples and detection result deviation, low detection result accuracy, cumbersome determination procedures, etc., to improve work efficiency, facilitate rapid detection, and reduce sample distillation The effect of the process
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Embodiment 1
[0022] Embodiment 1: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:
[0023] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 25°C for 20h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;
[0024] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , put it into a boiling water bath for reflux saponification for 25min, titrate with sulfuric acid until the pH of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;
[0025] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterification power=(C 1 V...
Embodiment 2
[0026] Embodiment 2: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:
[0027] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 35°C for 30h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;
[0028] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , placed in a boiling water bath for reflux saponification for 25 to 35 minutes, titrated with sulfuric acid until the pH value of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;
[0029] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterificati...
Embodiment 3
[0030] Embodiment 3: a kind of detection method of distiller's yeast esterification power, it comprises the following steps:
[0031] S1. Sample pretreatment: Weigh the koji to be tested with a weight equivalent to 10g of dry koji, denote the weight as M, add 80ml of distilled water and 20ml of mixed solution, and incubate at 30°C for 24h; wherein, the mixed solution has a mass percent concentration of 5% caproic acid, 5% acetic acid, 5% lactic acid and absolute ethanol;
[0032] S2. Titration: Titrate the pretreated sample to pH 9.0 with sodium hydroxide, then accurately add excess sodium hydroxide, the concentration is C 1 Volume is V 1 , put it into a boiling water bath for reflux saponification for 30min, titrate with sulfuric acid until the pH of the solution is 9.0, and the concentration of sulfuric acid is C 2 Volume is V 2 ;
[0033] S3. calculation: adopt the following formula to calculate the esterification power of distiller's yeast, esterification power=(C 1 V...
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