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Novel process for gathering and purifying stilbene substances in dendrobium officinale

A Dendrobium candidum, a new process technology, applied in the field of extraction and purification of stilbenes in Dendrobium candidum, can solve the problems of low reproducibility of column chromatography enrichment, unsuitable for large-scale production, low extraction efficiency, etc. The effect of large-scale production, avoiding gelatinization and shortening the extraction time

Active Publication Date: 2016-12-14
江西九草铁皮石斛科技协同创新有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have low extraction efficiency and high energy consumption, so they are not suitable for large-scale production
Most of the traditional purification methods use column chromatography, such as macroporous resin column chromatography, the enrichment reproducibility of column chromatography is not high, and a large amount of organic solvent is required

Method used

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  • Novel process for gathering and purifying stilbene substances in dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take the dried medicinal material of Dendrobium officinale, crush it, pass through a 60-mesh sieve, weigh 200g, put the powder of Dendrobium officinale in an extraction tank, add 10 times the amount of petroleum ether to degrease the first tank of medicinal material, reflux for 5 hours, and degrease twice , divide the degreased medicinal materials in the first tank into three parts, put them into the first, second and third tanks respectively, add 8 times of ethyl acetate to the three tanks respectively, and carry out the first extraction, the second and third extractions Add 5 times the amount of ethyl acetate respectively, and the medicinal liquid extracted for the second time in the second tank is used as the solvent for the first extraction in the third tank. The solvent for the second extraction, that is, the solvent added for the Nth extraction of each tank is the extraction solution of the N+1th time of the previous tank, and the solvent added for the third extrac...

Embodiment 2

[0032]Take the dried medicinal material of Dendrobium candidum, crush it, pass through a 60-mesh sieve, weigh 100g, put the powder of Dendrobium candidum in the extraction tank, add 10 times the amount of petroleum ether to degrease the first tank of medicinal material, after degreasing twice, put the first The medicinal materials in the pot are divided into three parts on average, and placed in three extraction pots respectively. Add 6 times the amount of ethyl acetate to the three cans for the first extraction, add 4 times the amount of ethyl acetate for the second and third times respectively, and the second and third cans of medicinal materials are supplemented on the basis of the previous can of medicinal liquid 1.5 times the amount of solvent; each can of medicinal materials was extracted 3 times, each extraction time was 1.5 hours, and 9.8g of crude extract was obtained; the crude extract was fully suspended with sodium hydroxide solution, stirred and reacted for 3 hours...

Embodiment 3

[0034] Take the dried medicinal material of Dendrobium officinale, crush it, pass through a 60-mesh sieve, weigh 100g, put the powder of Dendrobium officinale in a 2-liter round bottom flask, use 10 times the amount of petroleum ether to reflux in a water bath at 90°C for two times, each time for 3 hours, and put the petroleum ether After degreasing the medicinal materials, filter them back into a 2-liter round-bottomed flask, add 600 mL of ethyl acetate, and extract 3 times under reflux in a water bath at 90°C, each time for 3 hours, to obtain 6.8 grams of crude extract; L sodium hydroxide solution was fully suspended, stirred and reacted for 3 hours; the activated carbon powder was added to the sodium hydroxide solution, and continued to stir and fully adsorbed for 3 hours. The amount of activated carbon added was 8 times W / V of the material. hours, suction-filter the supernatant, then wash with 6 times the amount of water, centrifuge or suction-filter, and combine the supern...

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Abstract

The invention discloses a novel process for extracting, gathering and purifying stilbene substances in dendrobium officinale. The stilbene substances are important characteristic components in the dendrobium officinale and include phenanthrenes and bibenzyls. By the aid of the novel process, the problem that stilbene is obvious in biological activity but effective, simple and convenient processes for extracting and purifying the stilbene are unavailable at present can be solved. The novel process for extracting and purifying the stilbene substances in the dendrobium officinale is simple and is high in efficiency, and large-scale production can be facilitated. The novel process include main procedures of petroleum ether degreasing, tank assembly dynamic countercurrent ethyl acetate extraction, sodium hydroxide alkalization, hydrochloric acid acidification treatment and the like. Compared with the traditional macroporous resin purification process, the novel process has the advantages that large quantities of organic solvents and time can be saved, and the novel process is high in specificity; the purity of the stilbene substances can reach 80%.

Description

technical field [0001] The invention relates to the field of extracting effective plant components, in particular to a process for extracting and purifying stilbenes in Dendrobium candidum by means of a dynamic countercurrent method for extraction, alkali dissolution and acid precipitation. Background technique [0002] Dendrobium officinale is a perennial herb of the genus Dendrobium orchidaceae. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum is recorded in the 2015 edition of "Chinese Pharmacopoeia". Dendrobium officinale is a precious plant resource for both medicine and food. Dendrobium candidum is named for its iron-green skin. It is the best product of Dendrobium. Its stems are used as medicine. The tender stems are twisted into spirals or springs and dried in the sun. The commodity is called Dendrobium earrings, also known as Fengdou. Yin tonic. From the perspective of traditional Chinese medicine, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02A61K36/8984
CPCA61K36/8984A61K2236/33B01D11/02B01D11/0284B01D2011/002
Inventor 黄丽莉陈金龙朱培林王国鑫何彬宋喜红
Owner 江西九草铁皮石斛科技协同创新有限公司
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