Antifungal composition containing misulrom and fluzinm and application thereof
A technology of indazolamide and composition, applied in the directions of application, fungicide, biocide, etc., achieves the effects of delaying the occurrence and development, reducing the dosage and cost per mu, and improving the disease prevention effect
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Embodiment 1
[0050] Virulence Determination Example 1 Indoor Toxicity Determination Test on Potato Late Blight
[0051] Test object: Potato infestans ( Phytophthora infestans (Mont.) de Bary )
[0052] Test method: refer to the standard method of bioassay NY / T 1156.2-2006, using the medium method containing medicine: take 3mL of the medicine solution with a series concentration of each single agent and mixed agent, add it to 27mL PDA medium cooled to 45°C, and prepare Prepare the drug-containing medium plate at the desired final concentration. Then, a 6 mm-diameter mycelium block was prepared from the edge of the P. infestans colony cultured for 7 days, and moved to each series of drug-containing medium with the mycelium facing down, and each treatment was repeated 4 times. After the treatment, culture in a constant temperature biochemical incubator at 25°C, measure the colony diameter after 4 days, and calculate the growth inhibition rate. Through the linear regression analysis betwee...
Embodiment 2
[0061] Toxicity Determination Example 2 Indoor Toxicity Determination Test on Tobacco Black Leg Disease
[0062] Subject: Tobacco black shank (Phytophtora parasitica var. nicotianae Tucker)
[0063] Test method: refer to the standard method of bioassay NY / T 1156.2-2006, using the medium method containing medicine: take 3mL of the medicine solution with a series concentration of each single agent and mixed agent, add it to 27mL PDA medium cooled to 45°C, and prepare Prepare the drug-containing medium plate at the desired final concentration. Then, a 6 mm-diameter mycelial block was prepared from the edge of the tobacco black shank colony cultured for 7 days, and moved to each series of drug-containing medium, with the mycelium facing down, and each treatment was repeated 4 times. After the treatment, culture in a constant temperature biochemical incubator at 25°C, measure the colony diameter after 4 days, and calculate the growth inhibition rate. Through the linear regressi...
Embodiment 3
[0072] Toxicity Determination Example 3 Indoor Toxicity Determination Test to Chinese Cabbage Clubroot
[0073] Test object: clubroot of Chinese cabbage
[0074] Test method: refer to the standard method of bioassay NY / T 1156.1-2006, adopt the test of inhibiting the germination of pathogenic fungus spores (concave slide method): the spore suspension is prepared, and the cultured Chinese cabbage clubroot spores are cultured with deionized water from Elute on the base, filter, centrifuge for 5 minutes, pour off the supernatant, add deionized water, and centrifuge again. Finally, the spores were resuspended to 1 × 10 in deionized water. 5 ~1×10 7 spores and added 0.5% glucose solution. Use a pipette to prepare medicaments of different concentrations from low concentration to high concentration, draw 0.5ml of the medicinal solution at a time and add them to small test tubes, and then draw 0.5ml of the prepared spore suspension to make the medicinal solution and spore suspension...
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