Card type protein chip and application thereof

Abstract

The invention relates to the field of biological detection, and discloses a card type protein chip and an application thereof. A protein detecting method of inverse diffusion flow guide chromatography is designed from bottom to top and based on the vertical direction, samples to be detected and reaction reagents are diffused and subjected to chromatography in porous media and finally uniformly reach reaction films to perform immune reaction, precision becoming poor and missed diagnosis probabilities caused by non-uniform reaction are reduced, the reasonably designed card type protein chip can flexibly select treating and sample adding modes of markers, additives and substrates, a functional pad is designed, extension detecting types of filter pads, marker pads and auxiliary reaction pads are increased, detection agents can be added through a result interpretation window, and a reaction system can be adjusted and can avoid interference between different reactants and reach an optimal state.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a cassette protein chip and its application. Background technique [0002] As an emerging technology for multi-indicator parallel detection developed in recent years, protein chip technology has the characteristics of high throughput and high information content, and has broad application prospects in the fields of scientific research and clinical diagnosis. However, due to technical and cost factors, its practical application is limited, especially in the field of clinical diagnosis, which has not been promoted as it should. At present, a low-density protein chip that is low-cost, does not require complex and expensive equipment, is sensitive and accurate, can quickly perform multiple detections, and is easy to operate and is suitable for rapid point-of-care testing (POCT) is an important research and development direction in the field of protein chips. [0003] In the past ...

AI Technical Summary

Problems solved by technology

[0004] At present, this type of protein chip product based on immunochromatography technology, diafiltration method, and spot method technology, targeting POCT applications, has made great progress in technology and application, but there are still the following shortcomings: (1 ) Product quality is relatively easy to fluctuate, and it is not easy to carry out quality control; (2) The same product detection is mainly based on a single target, and at most 2-3 indicators can be detected at the same time, and multi-target detection is prone to mutual interference; (3) The experiments carried out Basically, only qualitative experiments can be carried out, and quantitative experiments cannot be carried out; (4) For multi-objective systems, it is difficult to adjust the production process and reaction system

However, traditional protein chips such as glass slides and silicon wafers are subject to many restrictions in application due to factors such as complex operations, high production requirements, and high costs.

Images