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Multiplex Magnetic-Activated Sorting Structural Microfluidic Chip with High Purity Cell Recovery

A microfluidic chip and magnetic activation technology, applied in the direction of stress-stimulated microbial growth, etc., can solve problems such as difficult alignment, complex alignment system, and low throughput, and achieve low price, high cell recovery rate, and reduced damage effect

Active Publication Date: 2021-04-02
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The system has the disadvantages of complex alignment system, difficult alignment and low throughput

Method used

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  • Multiplex Magnetic-Activated Sorting Structural Microfluidic Chip with High Purity Cell Recovery
  • Multiplex Magnetic-Activated Sorting Structural Microfluidic Chip with High Purity Cell Recovery
  • Multiplex Magnetic-Activated Sorting Structural Microfluidic Chip with High Purity Cell Recovery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This embodiment relates to a microfluidic chip with multiple magnetic activation sorting structures for high-purity cell recovery, such as figure 1 As shown, it consists of an inlet unit 1 , a first-stage magnetically activated sorting unit 2 , a second-stage magnetically activated sorting unit 3 and an outlet unit 4 connected in sequence.

[0057] The inlet unit 1 includes two inlets, which are cell suspension inlet 5 and buffer solution inlet 6 respectively.

[0058] The first stage magnetic activation sorting unit 2 (such as image 3 As shown), it is composed of a first micro-channel 8 , a second micro-channel 7 , a soft magnetic local magnetic field enhancement structure 9 and a first magnet 10 . After the buffer solution is injected from the buffer solution inlet 6 , a part of the buffer solution flows into the second microchannel 7 , and another part of the buffer solution flows into the first liquid microchannel 8 . Under the action of the soft magnetic local m...

Embodiment 2

[0082] This embodiment relates to a microfluidic chip with multiple magnetically activated sorting structures for high-purity cell recovery. It is the same as Embodiment 1, except that the shape of the inlet of the inlet unit 1 is square, and the side length is 1mm; the outlet unit The shape of the inlet of 4 is square, and the side length is 1 mm; the material of the soft magnetic local magnetic field enhancement structure 9 is iron-nickel alloy, and the shape is a cuboid unilateral zigzag structure. Soft magnetic local magnetic field enhancement structure 9 sawtooth width W 1 100μm, high W 2 is 500 μm; the first magnet 10 and the second magnet 13 are square electromagnets with a side length of 10 mm.

[0083] Using the chip of this embodiment to separate cells, the cell recovery rate was 85.3%, and the purity was 86.2%.

Embodiment 3

[0085] This embodiment relates to a microfluidic chip with multiple magnetically activated sorting structures for high-purity cell recovery, which is the same as that of Embodiment 1, except that the material 16 used to prepare the microfluidic channel is polymethyl methacrylate The inlet shape of the inlet unit 1 is an equilateral hexagon with a side length of 0.8mm; the inlet shape of the outlet unit 4 is an equilateral hexagon with a side length of 0.8mm; the shape of the soft magnetic local magnetic field enhancement structure 9 is comb-like structure. Soft magnetic local magnetic field enhancement structure 9 comb teeth W 1 100μm, high W 2 500 μm; the first magnet 10 is a square permanent magnet with a side length of 10 mm; the second magnet 13 is a square electromagnet with a side length of 10 mm.

[0086] Using the chip of this example to separate cells, the cell recovery rate was 84.8%, and the purity was 85.6%.

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Abstract

The invention provides a micro-fluidic chip with multiple high-purity cell recovery magnetic activated and sorting structures. The chip comprises an inlet unit, a first-stage magnetic activated and sorting unit, a second-stage magnetic activated and sorting unit and an outlet unit which are sequentially connected. The first-stage magnetic activated and sorting unit comprises a first micro-channel, a second micro-channel and a first-stage magnet, and the first micro-channel is arranged between the second micro-channel and the first-stage magnet. The second-stage magnetic activated and sorting unit comprises a third micro-channel, a fourth micro-channel and a second-stage magnet, and the third micro-channel is arranged between the fourth micro-channel and the second-stage magnet. Compared with the prior art, the chip has the advantages of being high in integrity, easy to operate, high in cell recovery rate, high in cell sorting purity and simple in manufacturing process.

Description

technical field [0001] The invention relates to a microfluidic chip with multiple magnetic activation sorting structures for high-purity cell recovery, which is suitable for the fields of cell separation capture and culture analysis, clinical trials, medical diagnosis, etc., especially in the separation of specific The field of cell isolation, sorting and manipulation of cells. Background technique [0002] From ancient times to the present, human beings have been threatened by various diseases. Nowadays, AIDS, cancer and diabetes seriously threaten human health. Studies have shown that single-cell analysis of high-purity cell populations or specific cells has a very important contribution to the research and overcoming of major diseases such as AIDS, cancer and diabetes. For this reason, providing a cell sorting method and chip with high-purity cell recovery that is convenient and fast will greatly promote the development of cell biology. Therefore, the technique of isol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
Inventor 陈迪林树靖郅晓仇三铭韦黔崔大祥
Owner SHANGHAI JIAOTONG UNIV
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