Extraction liquid for synchronously extracting flavone and polysaccharide of taxus chinensis and method thereof

A simultaneous extraction technology of yew flavonoids, which is applied to pharmaceutical formulas, medical preparations containing active ingredients, and medical raw materials derived from gymnosperm subphylums, etc., to achieve the effects of easy reuse, high extraction efficiency, and low viscosity

Inactive Publication Date: 2016-12-07
INST OF APPLIED CHEM JIANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The domestic and foreign studies on yew mainly focus on the development and utilization of paclitaxel, while the research on yew polysaccharides and flavonoids is less

Method used

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  • Extraction liquid for synchronously extracting flavone and polysaccharide of taxus chinensis and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for synchronously extracting taxus flavonoids and polysaccharides, said method comprising the following steps:

[0032] (1) Sample pretreatment: crush the dried southern yew branches and leaves to a 60-mesh sieve, add petroleum ether, reflux and degrease in a Soxhlet extractor for 3 hours, collect yew samples, and dry them for later use.

[0033] (2), ionic liquid-aqueous two-phase extraction: the yew sample after degreasing, ionic liquid [C 2 mim] Cl, K 2 HPO 4 and water are mixed in a weight ratio of 1:7.5:9:20 to form an ionic liquid-aqueous two-phase system, and the yew flavonoids and polysaccharides are extracted under the synergistic action of ultrasound and microwave. The microwave power is 300W, the microwave frequency is 2450MHZ, the ultrasonic power is 200W, the ultrasonic frequency is 25KHZ, and the synergistic time is 10min. After the extraction is complete, centrifuge and let it stand still. After the interface is stable, separate the upper and ...

Embodiment 2

[0037] A method for synchronously extracting taxus flavonoids and polysaccharides, said method comprising the following steps:

[0038] (1) Sample pretreatment: crush the dried yew branches and leaves to 80-mesh sieve, add petroleum ether, reflux and degrease in a Soxhlet extractor for 4 hours, collect yew samples, and dry them for later use.

[0039] (2), ionic liquid-aqueous two-phase extraction: the yew sample after degreasing, ionic liquid [C 3 mim] NO 3 , NaH 2 PO 4 and water are mixed in a weight ratio of 1:10:15:40 to form an ionic liquid-aqueous two-phase system, and the yew flavonoids and polysaccharides are extracted under the synergistic action of ultrasound and microwave. The microwave power is 600W, the microwave frequency is 2450MHZ, the ultrasonic power is 400W, the ultrasonic frequency is 25KHZ, and the synergistic time is 15min. After the extraction is complete, centrifuge and let it stand still. After the interface is stable, separate the upper and lower ...

Embodiment 3

[0043] A method for synchronously extracting taxus flavonoids and polysaccharides, said method comprising the following steps:

[0044] (1) Sample pretreatment: crush the dried yew branches and leaves to a 60-mesh sieve, add petroleum ether, reflux and degrease in a Soxhlet extractor for 3.5 hours, collect yew samples, and dry them for later use.

[0045] (2), ionic liquid-aqueous two-phase extraction: the yew sample after degreasing, ionic liquid [C 4 mim] CF 3 COO, K 2 CO 3and water are mixed in a weight ratio of 1:8:12:30 to form an ionic liquid-aqueous two-phase system, and the yew flavonoids and polysaccharides are extracted under the synergistic action of ultrasound and microwave. The microwave power is 450W, the microwave frequency is 2450MHZ, the ultrasonic power is 300W, and the ultrasonic frequency is 25KHZ, and the synergistic time is 12min. After the extraction is complete, centrifuge and let it stand still. After the interface is stable, separate the upper and...

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Abstract

The invention belongs to the technical field of extraction and separation of flavone and polysaccharide active ingredients in plants, and particularly relates to an extraction liquid for synchronously extracting flavone and polysaccharide of taxus chinensis and a method thereof. The method comprises the following steps of using crushed branches and leaves of taxus chinensis as raw material; under the synergetic action of ultrasonic wave and microwave, extracting and separating flavone and polysaccharide by a novel double-water phase system which is built by ionic liquid, inorganic salt and water; extracting and separating a flavone compound from an upper ionic liquid phase by an organic solvent, and recycling the ionic liquid and the organic solvent; obtaining a polysaccharide compound from a lower inorganic salt water phase by dialysis, and alcohol precipitation. The method has the advantages that the extraction efficiency is high, the separation condition is moderate, the green and environment-friendly effects of extracting solvent are realized, the recycling degree is high, and the like; the method is suitable for industrialized synchronous separation of flavone and polysaccharide compounds.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of active components of flavonoids and polysaccharides in plants, and in particular relates to an extraction solution for synchronously extracting yew flavonoids and polysaccharides and an extraction method thereof. Background technique [0002] Yew, also known as yew, yew, etc., is listed as a rare tree species in the world for protection because of its scarce resources, and is a rare and endangered species in my country. The domestic and foreign studies on yew mainly focus on the development and utilization of paclitaxel, while the research on yew polysaccharides and flavonoids is less. At present, more than 400 compounds have been isolated from Taxus chinensis, mainly divided into taxanes and non-taxanes. Taxanes are taxane diterpene compounds including paclitaxel, and non-taxane compounds are mainly triterpenoids, steroids, flavonoids and phenolic compounds. [0003] The io...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/13C08B37/00
CPCA61K36/13A61K2236/15A61K2236/331A61K2236/35A61K2236/53C08B37/0003Y02P20/54
Inventor 熊伟李雄辉王慧宾付建平胡居吾韩晓丹徐国良邓朝阳
Owner INST OF APPLIED CHEM JIANGXI ACAD OF SCI
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