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A method for preparing ribonucleotides from beer waste yeast sludge and feed additives containing ribonucleotides

A ribonucleotide and feed additive technology, applied in animal feed, animal feed, fermentation, etc., can solve the problems of long enzymatic hydrolysis time, limited ribonucleic acid content, and low hydrolysis efficiency, so as to improve the reproductive performance of female animals, The effect of promoting intestinal development and fast enzymatic hydrolysis

Active Publication Date: 2017-06-27
味氏(广东)生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing method for separating ribonucleic acid from beer waste yeast slime, the ribonucleic acid content obtained is very limited, about 5-6% (orcinol ribonucleic acid assay), the ribonucleic acid enzymatic hydrolysis method is very low in hydrolysis efficiency, and it takes a long time Enzyme hydrolysis time, about 22-30 hours

Method used

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  • A method for preparing ribonucleotides from beer waste yeast sludge and feed additives containing ribonucleotides
  • A method for preparing ribonucleotides from beer waste yeast sludge and feed additives containing ribonucleotides
  • A method for preparing ribonucleotides from beer waste yeast sludge and feed additives containing ribonucleotides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method for preparing ribonucleotides from beer waste yeast slime, comprising the following steps:

[0028] S1, preprocessing

[0029] Remove impurities from the waste beer yeast slime, then centrifuge at 1000r / min, remove the supernatant, and obtain a beer yeast slime concentrate with a dry matter of about 15%, so as to increase the dry matter concentration, thereby improving production efficiency. In this embodiment, fresh beer waste yeast sludge is used without deodorization treatment.

[0030] S2, the first enzymatic hydrolysis

[0031] Use NaOH solution to adjust the pH value of the beer yeast slime concentrate to 6.0; then add the first compound enzyme wherein, the first compound enzyme is made up of neutral protease and cellulase, and the weight ratio of the two enzymes is 1:1. The total weight is 1‰ of the total dry matter of the substrate, and the ratio of the enzyme activity to the substrate concentration is 5000U / g; then, the enzymolysis is car...

Embodiment 2

[0041] A preparation method for preparing ribonucleotides from beer waste yeast slime, comprising the following steps:

[0042] S1, preprocessing

[0043] Remove impurities from the waste beer yeast slime, then centrifuge at 1000r / min, remove the supernatant, and obtain a beer yeast slime concentrate with a dry matter of about 20%. In this embodiment, fresh beer waste yeast sludge is used, and deodorization treatment may not be carried out.

[0044] S2, the first enzymatic hydrolysis

[0045]Use NaOH solution to adjust the pH value of the beer yeast slime concentrate to 6.0; then add the first compound enzyme wherein, the first compound enzyme is made up of neutral protease and cellulase, and the weight ratio of the two enzymes is 1:1. The total weight is 2‰ of the total dry matter of the substrate, and the ratio of the enzyme activity to the substrate concentration is 6000U / g; then, the enzymatic hydrolysis is carried out at a temperature of 60°C and a pH of 6.0 for 15 hour...

Embodiment 3

[0054] A preparation method for preparing ribonucleotides from beer waste yeast slime, comprising the following steps:

[0055] S1, preprocessing

[0056] Remove impurities from beer waste yeast slime, then centrifuge at 1000r / min, remove the supernatant, and obtain a beer yeast slime concentrate with a dry matter of about 25%, so as to increase the dry matter concentration. In this embodiment, fresh beer waste yeast sludge can be used for deodorization treatment.

[0057] S2, the first enzymatic hydrolysis

[0058] Use NaOH solution to adjust the pH value of the beer yeast slime concentrate to 6.0; then add the first compound enzyme wherein, the first compound enzyme is made up of neutral protease and cellulase, and the weight ratio of the two enzymes is 1:1. The total weight is 3‰ of the total dry matter of the substrate, and the ratio of the enzyme activity to the substrate concentration is 8000U / g; then, the enzymatic hydrolysis is carried out at a temperature of 60°C an...

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Abstract

The invention relates to a method for preparing ribonucleotide from waste beer yeast paste and a feed additive containing the ribonucleotide. The preparation method comprises the following steps: S1, pretreatment; S2, primary enzymolysis: heating yeast paste obtained in the step S1 to 40-60 DEG C, regulating the pH value to 4.0-11.0, and then adding a first complex phosphoesterasum for enzymolysis to obtain a first enzymatic hydrolysate; S3, ultrafiltration and purification of a nucleic acid; S4, secondary enzymolysis: adding a second complex phosphoesterasum into a concentrated solution of ribonucleic acid for enzymolysis, and obtaining a second enzymatic hydrolysate; carrying out spray-drying to obtain a yeast nucleotide powder, wherein the monomer content of the nucleotide is 40-60wt%. The feed additive comprising the ribonucleotide comprises the nucleotide powder. The ribonucleotide is prepared from the waste beer yeast paste through twice enzymolysis, the enzymolysis of the beer yeast and the hydrolysis of the ribonucleic acid are speeded up through the set specific combination enzyme and the conditions, and the enzymolysis efficiency of the ribonucleotide is improved.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a method for preparing ribonucleotides from beer waste yeast slime and a ribonucleotide-containing feed additive. Background technique [0002] There are as many as 50,000 tons of waste yeast eliminated from beer production in my country every year, and the ribonucleic acid (RNA) content in beer yeast cells is as high as 4% to 6% of yeast cells. Nucleotides have been used in feed and aquaculture as feed additives. Many mammals, poultry, fish and shrimp, in their rapid growth stage, or when they encounter drastic changes in environmental and living conditions, endogenous nucleotides in many important immune organs of the body, such as lymph, bone marrow, intestinal tract, red blood cells and white blood cells, cannot Satisfy the needs, cause the body's immunity to drop sharply, and induce many diseases. Appropriate supplementation of exogenous nucleotides at the corresponding stage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/153A23K10/14C12P19/30
CPCC12P19/30
Inventor 江瑞华
Owner 味氏(广东)生物科技股份有限公司
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