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Detection method for total content of chitosan oligosaccharides with polymerization degree of 2 to 6

A technology of chitosan oligosaccharide and detection method is applied in the field of detection of the total content of chitosan oligosaccharide with 2 to 6 degrees of polymerization, which can solve the problem of no detection method for the total content of chitosan oligosaccharide, so as to enrich product types and meet health needs , the effect of technological improvement and promotion of healthy development

Inactive Publication Date: 2016-11-23
QINGDAO KEHAI BIOLOGICAL
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Problems solved by technology

[0004] However, there is no detection method for the total content of chitosan oligosaccharides with a degree of polymerization of 2 to 6 in the prior art.

Method used

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Embodiment 1

[0020] In the present invention, the detection method of the total content of chitosan oligosaccharides with 2 to 6 degrees of polymerization, the specific steps are as follows:

[0021] 1. Sample handling

[0022] (1) The standard substances chitobiose, chitotriose, chitotetraose, chitopentaose and chitohexaose were respectively prepared into standard solutions with concentrations of 2%, 2%, 4%, 8%, and 16%, and passed through 0.22 μm Filter through a microporous membrane, refrigerate at 4°C, and set aside;

[0023] (2) The chitosan oligosaccharide sample is prepared into a sample solution with a concentration of 30%, filtered through a 0.22 μm filter membrane, refrigerated at 4° C., and set aside;

[0024] 2. Separation and detection by high performance liquid chromatography

[0025] The high-performance liquid chromatograph is equipped with a differential refractive index detector, the mobile phase is acetonitrile: water = 73:27 (v / v), the flow rate of the mobile phase is...

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Abstract

The invention discloses a detection method for a total content of chitosan oligosaccharides with a polymerization degree of 2 to 6. The detection method comprises the steps that firstly chitosan oligosaccharides standard substances (chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose) and a chitosan oligosaccharides sample are matched to have an appropriate concentration, and injected into a high performance liquid chromatograph so as to be analyzed by high performance liquid chromatography; subsequently, the peak retention time and the peak area of the chitosan oligosaccharides standard substances are recorded, and the peak retention time and the peak area of the chitosan oligosaccharides sample are also recorded, thereby the chitosan oligosaccharides with the polymerization degree of 2 to 6 corresponding to each peak in the sample are determined by comparison with the peak retention time of the standard substances; finally, the concentration of the chitosan oligosaccharides with the polymerization degree of 2 to 6 in the sample is calculated from the ratio of the concentration to the peak area of the standard substances and the peak area of chitosan oligosaccharides with the polymerization degree of 2 to 6 in the sample, so that the total content of the chitosan oligosaccharides with the polymerization degree of 2 to 6 is calculated by the ratio of the concentration of the chitosan oligosaccharides with the polymerization degree of 2 to 6 to the sample preparation concentration. The detection method for the total content of the chitosan oligosaccharides with the polymerization degree of 2 to 6 fills the blank of content detection of chitosan oligosaccharides in the industry.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for detecting the total content of chitosan oligosaccharides with a polymerization degree of 2 to 6. Background technique [0002] With the development of economy and society and the improvement of living standards, people's pursuit of health has also increased, and healthy and safe functional foods are increasingly favored by consumers. [0003] Oligochitosan is a biopolymer obtained by enzymatic hydrolysis of chitosan, and is a functional polysaccharide composed of glucosamine as sugar units. Oligochitosan is the only positively charged alkaline polysaccharide in nature. It has a stable three-dimensional structure, has many beneficial functions on the human body, and has a wide application prospect. It can enhance the absorption of calcium and minerals, selectively proliferate probiotics, inhibit the growth of pathogenic bacteria, eliminate oxygen negative ion free radic...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 李悦明夏秀梅辛跃强赵常有徐建春
Owner QINGDAO KEHAI BIOLOGICAL
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