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Elephant grass vascular tissue-specific promoter and its application

A tissue-specific, promoter-based technology, applied in the field of plant biology, can solve problems affecting ruminants and unfavorable livestock weight gain

Active Publication Date: 2017-10-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in elephant grass, the lignin content is too high, which affects the digestion rate of ruminants, such as cattle, which is not conducive to livestock weight gain

Method used

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  • Elephant grass vascular tissue-specific promoter and its application
  • Elephant grass vascular tissue-specific promoter and its application
  • Elephant grass vascular tissue-specific promoter and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1 material

[0040] 1.1 Plant material: Elephant grass was taken from the introduction garden of the Grassland Science Department of South China Agricultural University.

[0041] 2 methods

[0042] 2.1 Acquisition of vascular tissue-specific expression promoter

[0043]According to the operating instructions of the Plant Genomic DNA Extraction Kit, Elephant Grass Genomic DNA was extracted, and the hi-TAIL PCR method was used to detect the 2054bp promoter sequence upstream of PpCAD (Elephant Grass Cinnamon Alcohol Dehydrogenase) gene ATG (named as the promoter region of PpCADPRO). ) for cloning, using 4 upstream degenerate primers LAD (1 / 2 / 3 / 4), 1 upstream universal primer AC1 and 3 downstream co-direction specific primers Hi-CADR1-0, Hi -CADR1-1 and Hi-CADR1-2 were subjected to three rounds of nested PCR amplification. According to the sequence obtained by the first hi-TAIL PCR, three specific downstream primers Hi-CADR2-0, Hi-CADR2-1 and Hi-CADR2-2 near the 5' end w...

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Abstract

The invention discloses a grassiness fibrovascular tissue specific promoter and an application thereof. The promoter is selected from any sequences as follows: (a) containing nucleotide sequence shown in any of SEQ ID No.1-9 or nucleotide sequence complementary with the sequence shown in any of SEQ ID No.1-9; (b) in the case of artificial decoration, the nucleotide sequence shown in any of SEQ ID No.1-9 has at least 75% of homologous nucleotide sequences. The promoter can be applied to the expression of specific plant tissue, so that effective manner and tool are supplied for the transgenosis breeding of the plants.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to an elephant grass vascular tissue-specific promoter and application thereof. Background technique [0002] Promoter refers to a DNA sequence that can interact with RNA polymerase and initiate transcription. It is called the hub of regulating gene expression, and it can control the expression level, expression site and expression mode of genes. In order to overcome constitutive promoters, such as cauliflower mosaic virus 35S promoter (CaMV 35s promoter), actin promoter (Actin promoter) and corn ubiquitin promoter (Ubiquitin promoter), when they are continuously and efficiently expressed in plants, they must Adverse effects on growth and development of plants. The research and application of tissue-specific or inducible promoters have attracted more and more attention from scientific researchers. Through tissue-specific or inducible promoters, not only can the precise expression...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84C12N1/21A01H5/04C12R1/01
CPCC07K14/415C12N15/8205C12N15/8226
Inventor 解新明钟天秀唐然陈曙张向前
Owner SOUTH CHINA AGRI UNIV
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