Application of natural killer cells combined with antidepressants in improving depressive symptoms

A technology of natural killer cells and killer cells, which is applied in the field of cellular immunology and neurology, and can solve the problems that have not yet been reported on the application of NK cell immunotherapy

Active Publication Date: 2020-09-11
南京和乃安健康科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] It has been reported that under pathological conditions, NK cells can penetrate the blood-brain barrier, activate the activity of nerve cells, and participate in the regulation of nervous system diseases, but there are no reports or patents on the application of NK cell immunotherapy in improving depression symptoms

Method used

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  • Application of natural killer cells combined with antidepressants in improving depressive symptoms
  • Application of natural killer cells combined with antidepressants in improving depressive symptoms
  • Application of natural killer cells combined with antidepressants in improving depressive symptoms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] NK cells combined with fluoxetine improve the slow-moving mild unpredictable depression model in rats

[0020] (1) Experimental method:

[0021] Establishment of slow-moving mild unpredictable depression model in rats

[0022] 24 SPF grade SD rats, male, were randomly divided into 6 control groups and 18 model groups. The following stress factors were randomly applied to the rats in the model group within 3 weeks: ①Food and water for 24 hours; ②Water for 24 hours Food for 24 hours ③ Swimming at 4°C for 5 minutes ④ Tail clamping for 1 minute (hemostat is 1 cm from the base of the tail) ⑤ Restraint stress for 2 hours ⑥ Environment at 40°C for 5 minutes ⑦ Moist bedding and rat cage tilt (45°C) for 24 hours ⑧ Suspension for 5 minutes ⑨ Inversion of light and dark for 24 hours; Each stimulus was used twice on average, and each stimulus could not appear consecutively, so that the animals could not predict the occurrence of the stimulus.

[0023] Evaluation of a slow-moving ...

Embodiment 2

[0051] NK-92 cells combined with paroxetine improve depressive symptoms in patients after irradiation

[0052] NK-92 cell culture

[0053] The human NK-92 cell line was purchased from the Chinese Academy of Sciences Cell Bank / Stem Cell Bank, and the cells were resuspended in X-Vivo 10 serum-free medium containing 500U / ml human recombinant IL-2, 0.6mm L-aspartic acid, 3mm L- Glutamine, 1.8 mm L-serine and 2.5% human AB plasma, adjusted to a cell density of 2.5 x 10 5 / ml, 25ml cultured in 1L Vuelife culture bag, rehydration every 3 days to maintain cell density at 2.5×10 5 NK-92 cells were resuspended in normal saline for reinfusion (make sure to carry out fresh culture medium containing IL-2 48 hours before reinfusion) to reduce cell aggregation. rehydration), NK-92 cells were irradiated with 500cGy before reinfusion, and the survival status of NK-92 was detected (see NK-92 cell killing activity after irradiation). Figure 6 ), pending cell infusion.

[0054] NK-92 cells c...

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Abstract

The invention discloses a new application of natural killer (NK) cellular immunotherapy. An animal model is utilized to research the influence of NK cell in combination with fluoxetine for treating monoamine neurotransmitter, peripheral blood cell factor level and depression action in brain of a rat having chronic unpredictable depression; clinically, improvement of depression symptoms of a patient and adjustment of immunity index by irradiating NK-92 cell in combination with paroxetine are evaluated, and remarkable improvement on the depression symptom by an NK cell immunotherapy is proved. Therefore, the NK cell immunotherapy in combination with the antidepressant can remarkably improve the depression symptoms.

Description

technical field [0001] The invention relates to the technical fields of cellular immunology and neurology, in particular to the application of natural killer cells combined with antidepressants in improving depressive symptoms. Background technique [0002] Depression is an affective mental disorder syndrome characterized by persistent low mood. This disease has the characteristics of high incidence, difficult cure and high recurrence rate, which directly affects the working status and quality of life of patients. The monoamine neurotransmitter hypothesis is one of the widely accepted biological mechanisms for the pathogenesis of depression. Drugs developed based on this hypothesis are currently the main means of antidepressant treatment, including tricyclic antidepressants, monoamine oxidase inhibitors, 5 -HT and NA reuptake inhibitors, etc. However, clinical data show that patients need to take the drug for several weeks to take effect, some patients are prone to relapse,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K35/17A61P25/24
CPCA61K35/17A61K45/06A61K2300/00
Inventor 郑劼
Owner 南京和乃安健康科技有限公司
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