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Cultivating method of pleione bulbocodioides polyploidy plants

A cultivation method, the technology of solitary orchid, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problem of high content of medicinal active ingredients, achieve simple preparation method, significant polyploid characteristics, and high application value effect

Inactive Publication Date: 2016-11-23
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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Problems solved by technology

[0008] 3. High content of medicinal active ingredients

Method used

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  • Cultivating method of pleione bulbocodioides polyploidy plants
  • Cultivating method of pleione bulbocodioides polyploidy plants
  • Cultivating method of pleione bulbocodioides polyploidy plants

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Embodiment 1

[0040] 1) Take one Suanlan capsule, soak it in 0.1% washing powder solution for 10 minutes, and rinse it with tap water for 5 minutes. Then disinfect with 75% ethanol for 2 minutes, rinse with sterile water, disinfect with 0.1% mercuric acid for 15 minutes, rinse the residual mercuric acid with sterile water, and dry the remaining water on the surface of the capsule with sterile absorbent paper;

[0041] 2) Cut off the capsule of Suanlan orchid, take the seeds in the capsule and put them into a 0.1% colchicine solution (with 1 / 2 MS as the solvent), and cultivate them in the dark for 7 days. After finishing the treatment, centrifuge at 1000rpm for 1min to precipitate the seeds, take the seeds, wash them three times with sterile distilled water, and obtain the preliminary germinated seeds of Orchid albicans;

[0042] 3) Transplant the above-mentioned colchicine-treated Orchid albicans seeds into the medium of MS+0.2mg / L 1-naphthaleneacetic acid, place them in an incubator for da...

Embodiment 2

[0056] The specific steps are as described in Example 1, except that the concentration of colchicine in step 2 is 0.20%, the treatment time is 9 days, and the variation rate is 41.07±1.34%.

[0057] The polyploid solitary orchid plant obtained by the present invention can significantly improve the weight of a single corm, and the plant morphological characteristics are: plant height 40-70 cm, scape length 15-40 cm, 1 leaf, length 35-55 cm, width 5 cm ~9cm. The capsule is 3-5cm long and 0.7-1.5cm in diameter. The average weight of the capsule is 1200-1800mg, the largest capsule is 2300mg, the weight of a single bulb is 25-45g, and the diameter is 3-5cm.

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Abstract

The invention provides a method for cultivating polyploid plants of Orchid oleifera, which comprises the steps of treating Orchid oleifera seeds with colchicine, germination and cultivation of Orchid solitary seeds, proliferation and cultivation of seedlings of Orchid solitary, and is identified as polyploid The ploidy plant is then transferred to the medium for rooting and strong seedlings and cultivated to obtain the seedlings of the polyploid plant; the polyploid plant of the polyploid plant that the present invention utilizes the seed of the polyploid to obtain, has remarkable The polyploid characteristics of the polyploid, and the preparation method is simple, and it has important guiding significance for the cultivation of new varieties of orchids. The polyploid of the orchids obtained by the invention has high application value.

Description

technical field [0001] The present invention relates to a method for cultivating orchid alone, in particular to a method for cultivating polyploid plants of orchid only. Background technique [0002] The dried pseudobulb of the orchid plant Pleione bulbocodioides (Franch.) Rolfe is one of the main sources of the traditional Chinese medicine Arrowhead. The 2010 edition of "Chinese Pharmacopoeia" records that the main sources of Arrowhead Arrowhead are the orchid plants Rhododendron orchid (Cremastra appendiculata (D.Don) Makino), only garlic orchid (Pleione bulbocodioides (Franch.) Rolfe) or Yunnan single garlic orchid (Pleione yunnanensis ( Dried pseudobulbs of Rolfe) Rolfe). The former is commonly called "Mao Cigu", and the latter two are commonly called "Hockey Ball", both of which are used as Shan Cigu. It is sweet in taste, slightly pungent, cool in nature, has the effects of clearing away heat and detoxification, reducing swelling and dispelling stagnation, and is mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/005
Inventor 丁志山钱朝东金丽霞黄燕芬黄伟鹏蒋福升孙桂芹
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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