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Microbiological strain for repairing heavy metal contaminated soil, and screening method and application thereof

A technology of polluted soil and microorganisms, applied in the field of remediation of heavy metal-contaminated soil microbial strains and its screening, can solve the problems of long remediation period, large amount of engineering, secondary pollution, etc., achieve strong bioremediation function, small soil disturbance, strong effect of tolerance

Active Publication Date: 2016-11-16
中国烟草总公司广西壮族自治区公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods often involve a large amount of engineering and high energy consumption; most chemical methods are expensive and easily cause secondary pollution; phytoremediation in biological methods is favored by many researchers, but the plants grow slowly and the repair period is long
At present, most of the restoration technologies that have received more attention are in the laboratory test stage, and the experimental results are not ideal. In the field restoration, there are no good successful cases in our country.

Method used

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  • Microbiological strain for repairing heavy metal contaminated soil, and screening method and application thereof
  • Microbiological strain for repairing heavy metal contaminated soil, and screening method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the screening of microbial strain of the present invention

[0030] Liquid-phase enrichment method: (1) Weigh an appropriate amount of cadmium-contaminated soil sample, add it to an Erlenmeyer flask containing LB liquid medium, add an appropriate amount of glass beads, shake the Erlenmeyer flask at 28~30°C, 160~170rpm, Obtain a soil mix.

[0031] (2) Transfer the soil mixture into a centrifuge tube, and take the centrifuged supernatant A as the source of cadmium inactivated microorganisms.

[0032] (3) Inoculate the supernatant A obtained in step (2) into the LB liquid medium containing cadmium, shake and cultivate at 30°C and 160-170rpm, after the cultivation is completed, centrifuge, and draw 1-2mL with a sterile pipette The supernatant B was transferred into another LB liquid medium containing cadmium, and transferred three times in this way, and the microbial strain was domesticated through the above-mentioned enrichment culture method, and the bacter...

Embodiment 2

[0034] Embodiment 2: the identification of microbial strain of the present invention

[0035] The cd-M DNA was extracted according to the steps of the TIANamp bacteria DNA kit (TIANamp bacteria DNA kit) for strain identification. In the result with the highest matching degree, the measured 16S rDNA sequence part of the strain has 100% homology with the 16S rDNA sequence of Bacillus (Bacillus), and it can be determined that the genus is Bacillus (Bacillus ). Among the matches with known species, there is 100% homology to the 16S rDNA sequence of Bacillus mycoides (Bacillus mycoides). It has the highest sequence similarity with Bacillus mycoides (Bacillus mycoides) in the RDP-II database (S_ab score: 1.000). The bacterial species can be identified as Bacillus mycoides (Bacillus mycoides).

Embodiment 3

[0036] Embodiment 3: the growth curve of microbial strain of the present invention

[0037] The strains were inoculated in LB liquid medium, the inoculum amount was 0.5%-1%, shake culture at 160-170rpm at 30°C, and samples were taken intermittently. Such as figure 1 As shown, at 4 hours, it enters the logarithmic growth phase, and at 10 hours it reaches the stable phase. After 25 hours, the growth tends to decline, and it enters the decay phase. The highest concentration can reach OD 600 is 5.34.

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Abstract

The invention relates to the field of microorganism, and discloses a microbiological strain for repairing heavy metal contaminated soil, and a screening method and application thereof. The preservation number of the microbiological strain disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 10127. According to the screening method disclosed by the invention, a bacillus mycoides is screened from soil contaminated by heavy metal cadmium, exchangeable cadmium in the soil can be passivated by the bacillus mycoides, the content of the exchangeable cadmium can be obviously reduced, and meanwhile, a very strong tolerance on cadmium is also obtained; disturbance on the soil is small, a stronger biological repairing function is obtained, and the microbiological strain can be applied to repairing of the heavy metal contaminated soil and preparing of heavy metal contaminated repairing materials.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial strain for remediating heavy metal polluted soil, a screening method and application thereof. Background technique [0002] In recent years, my country's industrialization and urbanization have developed rapidly. The continuous expansion of industrial scale and the construction of housing, roads, railways and various public facilities have occupied a large amount of cultivated land, resulting in a sharp decrease in the cultivated land area. Moreover, a large amount of usable arable land has been polluted by industrial and mining sewage to irrigate farmland, large-scale application of pesticides and fertilizers, and stacking of solid waste, which has led to deterioration of environmental quality, erosion of green vegetation, and massive reduction in crop production or toxicity. Heavy metal polluted soil is concealed, cumulative, and hysteresis, and it is difficult to rec...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10C12N1/20C12N1/205C12R2001/07
Inventor 何声宝张威罗安娜刘楠王英元冯晓民林北森王五权韦忠高华军赖洪敏周文亮姚彬罗刚刘春萍韦陈鹰
Owner 中国烟草总公司广西壮族自治区公司
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